1 friday, april 19 Biochemistry and Molecular Biology asbmb graduate and postdoctoral travel award keynote lecture special Session



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C. Cramer and L. Chase. Hope Col., MI.

C256 


1017.9  Identification of putative trafficking motifs in 

System x


c

-



S. Lang, A. Georges and L. Chase. Hope Col., MI.

C257 


1017.10  Cell surface expression of xCT is regulated by 

the Akt signaling pathway. 



L. Chase, D. Smith and N. Schiller. 

Hope Col., MI.

C258 

1017.11  Vitamin E and nonalcoholic fatty liver disease. 

V. Thakur, L. Ulatowski, R. Parker, R. Zhang, X. Liu and 

D. Manor. Case Western Reserve Univ., Cornell Univ. and 

Cleveland Clin. Fndn.

C259 

1017.12  Global deletion of stearoyl-CoA desaturase-2 

enhances glucose tolerance. 



L. O’Neill and J. Ntambi. Univ. of 

Wisconsin-Madison.

C260 

1017.13  Characterization of the role of long chain acyl-

CoA synthetase 6 in skeletal muscle energy metabolism. 



S.Y. 

Bu. Kyungil Univ., South Korea.

C261 


1017.14  When fed a high-fat diet,GPAT4

-/-


 mice have 

an increased metabolic rate and impaired growth and weight 

gain. 

D. Cooper and R.A. Coleman. Univ. of North Carolina at 

Chapel Hill.



BIOCHEMISTRY TUESDAY

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1018. LIPID SECOND MESSENGERS

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C262 


1018.1  cAMP-stimulated expression of DGK

q requires 

steroidogenic factor-1 and sterol regulatory element binding 

protein. 



K. Cai and M. Sewer. Skaggs Sch. of Pharm. and 

Pharmaceut. Sci., UCSD.

C263 

1018.2  The basis of the substrate specificity of 

the epsilon isoform of human diacylglycerol kinase is not a 

consequence of competing hydrolysis of ATP. 

R.M. Epand, 

A. Prodeus, B. Berno and M.K. Topham. McMaster Univ., 

Canada and Univ. of Utah.

C264 

1018.3  Phospholipase C

b1 is linked to RNA 

interference of specific genes through translin-associated 

factor X. 



S. Scarlata, F. Philip and Y. Guo. Stony Brook Univ.

1019. LIPID SIGNALING

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C265 


1019.1  Characterization of tocopherol kinase activity 

in primary human coronary artery smooth muscle cells. 



J-M. 

Zingg, M. Meydani and A. Azzi. Tufts Univ.

C266 


1019.2  Expression and characterization of 

recombinant neutral sphingomyelinase 2 in Escherichia coli

evidence of sensitivity to redox state. 

P.P. Dotson II and M. 

Nikolova-Karakashian. Univ. of Kentucky.

C267 


1019.3  Searching the extracellular domain of CD36 for 

fatty acid binding by surface plasmon resonance. 



A. Jay, A. 

Chen and J.A. Hamilton. Boston Univ.

C268 


1019.4  The role of endosomal phosphoinositides in 

phagocytosis, phagosome maturation and the endosomal 

system in macrophages. 

G.H.E. Kim, M. Dayam and R. 

Botelho. Ryerson Univ., Canada.

C269 


1019.5  Vac14 multimerization is required for Fab1 

complex assembly and function. 



S.C.Y. Ho, T. Alghamdi and 

R.J. Botelho. Ryerson Univ., Canada.

C270 


1019.6  Membrane ultrastructure modulates immune 

cell signaling. 



A. Pralle, H. Huang and M. Simsek. Univ. at 

Buffalo.


1020. LIPID STORAGE

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C271 


1020.1  Cholesteryl ester transfer protein deficiency in 

adipocytes disrupts triglyceride metabolism in multiple ways. 



D.J. Greene and R.E. Morton. Lerner Res. Inst., Cleveland 

Clin.


C272 

1020.2  Both full-length and exon 9-deleted human 

cholesteryl ester transfer protein isoforms are involved in 

cellular lipid homeostasis. 

L. Izem and R.E. Morton. Lerner 

Res. Inst., Cleveland Clin.

C273 

1020.3  Characterization of the proteome of cytoplamic 

lipid droplets in enterocytes in response to dietary fat. 



T. 

D’Aquila, D. Sirohi, R.J. Kuhn and K.K. Buhman. Purdue 

Univ.


C274 

1020.4  Characterization of a perilipin 5 splice variant. 

T.C. Hubbell, D.M. DuBreuil and J.T. Tansey. Otterbein Univ., 

OH.


1021. PROTEIN-LIPID INTERACTIONS

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C275 


1021.1  Acrolein, a component of tobacco smoke and 

age-related oxidative stress disrupts structure and function 

of apolipoprotein E. 

T.N. Tran, Y.Y. Lee, K. Uchida and V. 

Narayanaswami. California State Univ., Long Beach, and 

Grad. Sch. of Agr. Sci., Nagoya Univ., Japan.

C276 

1021.2  Fatty acids and their thioester derivatives 

as potential endogenous ligands of LXR

a.  G.V. Hines, A. 

Kaliappan, S.D. Rider, Jr. and H.A. Hostetler. Boonshoft Sch. 

of Med., Wright State Univ.

C277 

1021.3  A characterization of the modular nature 

of histone-derived antimicrobial peptides. 



M.E. Bustillo, 

M.A. LaBouyer, A.L. Fischer, D.C. Webb and D.E. Elmore. 

Wellesley Col.

C278 

1021.4  A modular approach to the histone H2A family 

of antimicrobial peptides. 



A.L. Fischer, M. Bustillo and D.E. 

Elmore. Wellesley Col.

C279 


1021.5  Engineering a specific phosphatidylcholine 

binding site motif into a phosphatidylinositol-specific 

phospholipase C. 

R. Goldstein, J. Cheng, B. Stec, A. 

Gershenson and M.F. Roberts. Boston Col., Sanford-

Burnham Med. Res. Inst., La Jolla and Univ. of Massachusetts 

Amherst.

C280 


1021.6  Role of arginine and lysine in the antimicrobial 

mechanism of histone-derived peptides. 



K. Cutrona and D. 

Elmore. Wellesley Col.

C281 


1021.7  Biochemical analysis unveils the mechanism 

of the interaction between Hsp70s and lipids. 



C. McCallister 

and N. Nikolaidis. California State Univ., Fullerton.

C282 


1021.8  Spatial and temporal regulation of the Nedd4 

family ubiquitin ligases through phospholipid binding. 



J. Scott 

and R.V. Stahelin. Univ. of Notre Dame and Indiana Univ. Sch. 

of Med. South Bend.

C283 

1021.9  Lipid binding properties of Ebola virus matrix 

protein VP40. 



S. Yong, J.L. Scott and R.V. Stahelin. Univ. of 

Notre Dame and Indiana Univ. Sch. of Med. South Bend.

C284 

1021.10  The interaction of Hsp70s and lipids is 

conserved from bacteria to humans. 



M.C. Siracusa and N. 

Nikolaidis. California State Univ., Fullerton.

C285 


1021.11  Expression and purification of a MORN fusion 

protein from Pseudomonas aeruginosa



C.C. Anderson, L.C. 

MacDonald, J.B. Nice, B.W. Berger and C.N. Streu. St. 

Mary’s Col. of Maryland and Lehigh Univ.



TUESDAY BIOCHEMISTRY

340

1022. STRESS AND LIPID METABOLISM

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C286 


1022.1  Modulating lipid fate controls lipotoxicity in 

palmitate-treated hepatic cells. 



A. Leamy, M. Shiota and J. 

Young. Vanderbilt Univ.

C287 


1022.2  Protective effects of coenzyme Q10 on 

dystrophic muscle cells. 



E. Minatel, D.S. Mizobuti, A.B. 

Macedo and L.H.R. Moraes. Univ. of Campinas, Brazil.

1023. STRUCTURAL BIOLOGY AND MECHANISMS OF 

MEMBRANE LIPID ASSEMBLY

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C288 


1023.1  Probing the structure and function of ApoA-I 

C-terminal domain during HDL formation. 



X. Mei and D. 

Atkinson. Boston Univ. Sch. of Med.

C289 


1023.2  Mechanisms of non-random sphingolipid 

organization in the plasma membranes of fibroblast cells. 



M.L. 

Kraft, H.A. Klitzing, J.F. Frisz, K. Kou, P.K. Weber and J. 

Zimmerberg. Sch. of Chem. Sci., Univ. of Illinois at Urbana-

Champaign, Lawrence Livermore Natl. Lab. and NICHD, NIH.



1024. LIPID DOMAINS AND LIPID RAFTS

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C290 


1024.1  The iron transport inhibitor ferristatin II induces 

degradation of transferrin receptor-1. 



S.L. Byrne, P.D. Buckett, 

J. Sanford, J. Chen, C. Enns and M. Wessling-Resnick. 

Harvard Sch. of Publ. Hlth. and Oregon Hlth. & Sci. Univ.

C291 

1024.2  Differential oligomeric nature of mEos2 and 

mEos3.2 fluorescent proteins is consequential to diffusion and 

confinement of membrane probes. 

E. Edwald and S.L. Veatch. 

Univ. of Michigan.



1025. MEMBRANE PROTEIN SYNTHESIS, INSERTION 

AND ASSEMBLY

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C292 


1025.1  Targeting of a mating machinery ATPase to 

the cell membrane. 



M.P. Broulidakis, S.J. Laurer and M.B. 

Berkmen. Suffolk Univ., MA.

C293 


1025.2  Structural insights into the role of BamA in the 

biogenesis of beta-barrel membrane proteins in Gram-negative 

bacteria. 

N. Noinaj, A. Kuszak, J.C. Gumbart, P. Lukacik, H. 

Chang, N. Easley, T. Lithgow and S.K. Buchanan. NIDDK, 

NIH, Georgia Tech, Diamond Light Source Ltd, Oxford, FDA, 

Rockville and Monarch Univ., Australia.

1026. PROTEIN-LIPID INTERACTIONS AS 

DETERMINANTS OF FUNCTION

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C294 


1026.1  Regulation of the ATP hydrolysis and transport 

cycles of the P-glycoprotein multidrug transporter by sterols 

and phospholipids. 

F.J. Sharom, A.T. Clay and P. Lu. Univ. of 

Guelph, Canada.

C295 

1026.2  Surprising phospholipid specificity of two blood 

clotting proteins: factor VII and protein C. 



N. Tavoosi and J.H. 

Morrissey. Univ. of Illinois at Urbana-Champaign.

C296 


1026.3  The role of cardiolipin in defining the mammalian 

ANT interactome. 



Y-W. Lu, M.G. Baile, T-C. Huang, A. Pandey 

and S.M. Claypool. Johns Hopkins Univ. Sch. of Med.

1027. CELL CYCLE

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C297 


1027.1  Cell cycle regulation mechanism in pathogenic 

yeast, Cryptococcus neoformans: structure-function 

relationship of G1 and G1/S cyclins homologue CnCln1. 

S. 

Kawamoto, E.V. Virtudazo, A. Suganami and Y. Tamura. 

Chiba Univ. Med. Mycol. Res. Ctr. and Grad. Sch. of Med., 

Japan.

C298 


1027.2  Video time lapse imaging of mitotic catastrophe 

events in response to thiopurines. 



S. Patel and D. Pittman. 

Univ. of South Carolina and South Carolina Col. of Pharm.

C299 

1027.3  Identification and characterization of mitotic 

defect-related proteins (MDr1 and MDr2) necessary for bipolar 

spindle assembly. 

K. Cheung and J. Torres. UCLA.

C300 


1027.4  The disruption of the mitotic cell cycle exit by 

inositolless death in S. cerevisiae



B.A. Hanson, P.O. O’Connor, 

J. Frandina, J. Pittari, J. Munezero and V. Croglio. Canisius 

Col., NY.

C301 

1027.5  Paradoxical effects of metformin on the cell 

cycle in L6 myoblasts. 



R. Parakhia and R.S. Ochs. St. John’s 

Univ., NY.

C302 

1027.6  Adiponectin receptor (ADIPOR1) signaling 

ameliorates obesity-dependent cell cycle entry in MCF7 cells. 



M. Connor and C. Theriau. York Univ., Canada.

C303 


1027.7  The ubiquitin conjugating enzyme UbcH10 

competes with UbcH3 for binding to the SCF complex, a 

ubiquitin ligase involved in cell cycle progression. 

A.Y. Le 

Feuvre, D. Afful, F. Shang and A. Taylor. USDA at Tufts Univ.

BIOCHEMISTRY TUESDAY


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C304 


1027.8  The SUMO ligase PIAS1 promotes the 

progression and survival of solid tumors of different 

histopathological origins. 

J.D. Constanzo, A. Rabellino, G. 

Konstantinidou, K. Schuster and P. Scaglioni. Univ. of Texas 

Southwestern Med. Ctr.



1028. CELL CYCLE AND GROWTH CONTROL

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C305 


1028.1  MED28 regulates cell growth through HMG 

box-containing protein 1 in human breast cancer cells. 



N-T. 

Hsieh, C-Y. Huang, Y-H. Chou, W.C. Ung and M-F. Lee. China 

Med. Univ. and Chang Jung Christian Univ., Taiwan.

C306 

1028.2  Cranberry proanthocyanidins affect human 

prostate cancer cell growth via cell cycle arrest by modulating 

expression of cell cycle regulators. 

R. Hurta, J. Kim, B. 

McKeown, K. Patel and C. Neto. Univ. of Prince Edward Isl., 

Canada and Univ. of Massachusetts Dartmouth.

C307 

1028.3  Artemisia princeps var. orientalis induces 

cell cycle arrest accompanied by upregulation of p21,

CIP1

 and 


caspase-dependent apoptosis in human breast carcinoma 

cells. 


E-J. Choi and G-H. Kim. Duksung Women’s Univ., South 

Korea.


C308 

1028.4  Functional analysis of the interaction between 

Kaposi sarcoma-associated herpes virus anti-apoptotic Bcl-

2 homolog and the human host pro-apoptotic protein, Bik. 

B. 

Caruso, S. Walker and J. Roecklein-Canfield. Simmons Col.

C309 


1028.5  Muscle enriched A-type lamin interacting 

protein a novel regulator cardiac growth and function. 



M-E. 

Cattin, E. Mak-Washburn and P.G. Burgon. Univ. of Ottawa 

Heart Inst.

C310 

1028.6  Effects of sulforaphane on three tumor cell 

lines and one normal cell line. 



F. Jin, T. Terwilliger, J. Bosch 

and R.W. O’Donnell. SUNY Geneseo.

C311 


1028.7  Using carboxyfluorescein succinimidyl ester 

to measure the effects of sulforaphane on cell division in a 

human leukemia cell line. 

K. Lehner, O. Feliciano and R.W. 

O’Donnell. SUNY Geneseo.

1029. CHECKPOINT MECHANISMS

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C312 


1029.1  Nuclear localized BMP2 promotes cell cycle 

progression. 



B.A. Nichols, W.A. Goar, B.T. McCune and L.C. 

Bridgewater. Brigham Young Univ.

1030. CYCLINS AND CYCLIN-DEPENDENT KINASES

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C313 


1030.1  The role of cyclin-dependent kinase 5 in insulin 

exocytosis. 



A. Trye and A. Aguanno. Marymount Manhattan 

Col.


C314 

1030.2  Pentoxifylline initiates GSK-3

b-induced 

proteasomal degradation of cyclin D1 and arrests renal cancer 

cells in the G1 phase. 



W. Cai, N.J. Mastrandrea, K.Y. Tham, 

T.J. Monks and S.S. Lau. Univ. of Arizona Col. of Pharm.

C315 


1030.3  Cyclin dependent kinase 5’s role in the 

development of both insulin-positive and neuronal phenotypes. 



D.E. Khost and A. Aguanno. Marymount Manhattan Col.

1031. MECHANISMS OF SIGNAL TRANSDUCTION II

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Session I scheduled on Monday.

C316 

1031.1  YAP mediates crosstalk between the Hippo 

and PI(3)K–TOR pathways by suppressing PTEN via miR-29. 



K.A. Tumaneng. UCSD.

C317 


1031.2  A-kinase anchoring protein-Lbc coordinates 

protein kinase A phosphorylation and inhibition of Src homology 

2 domain-containing phosphatase 2. 

B.T. Burmeister, D.M. 

Taglieri, L. Wang and G.K. Carnegie. Univ. of Illinois at 

Chicago.


C318 

1031.3  Specific mutations on the receptor-binding 

interface of non-visual arrestins differently affect recruitment 

by the NPY receptor family. 

L.E. Gimenez, S. Babilon, A.G. 

Beck-Sickinger and V.V. Gurevich. Vanderbilt Univ. Med. Ctr. 

and Leipzig Univ.

C319 

1031.4  Analysis of MILLIPLEX

®

 MAP NF



kB signaling 

using Luminex

®

 xMAP


®

 technology. 



J. Hwang, T. Warmke and 

R. Wiese. EMD Millipore, St. Charles, MO.

C320 


1031.5  Inhibition of ERK5 protects pulmonary fibrosis 

via downregulation of TGF-

b1-Smad signaling. S. Kim, S. Park, 


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