Appendix A. Supplementary material for online version



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Appendix A. Supplementary material for online version
SI Figure S1. Bayesian primary concordance trees of Myrtales using BUCKy. See Fig. 1 for comparison.

SI Figure S2. Ancestral range estimation (ARE) on the Myrtales chronogram with BioGeoBEARS (DEC+J model). Output shows the frequency (as a proportion of the pie) of each likely area (or combined area) at each node and corner. See Fig. 3 that shows only the dominant area (or combined area) at each node and corner.

SI Table S1. Species, voucher/collection information, and GenBank accession numbers.

SI Table S2. Primers used for amplification and sequencing of rbcL, matK, ndhF, 18S nrDNA, 26S nrDNA and matR.

Primer Sequence Reference
rbcL: Huang and Shi (2002)

PR1Fa 5’-ATG TCA CCA CAA ACA GAG ACT-3’

PR2Ra 5’-CCT TCA TTA CGA GCT TGC ACA C-3’

matK: This study

Comb1Fa 5’-TGT ATC GCA CTA TGT ATC ATT TG-3’

Comb2Ra 5’-TCC ATT AAG AGG CAT AAT TG-3’

Mel1Fa 5’-GCA ASA TAA CTT CCT ATA CC-3’

Mel2Ra 5’-GTY TCR ARC TTC TTC ATA G-3’

ndhF: Olmstead et al. (1993)

972Fa 5’-GTC TCA ATT GGG TTA TAT GAT G-3’

2110Ra 5’-CCC CCT AYA TAT TTG ATA CCT TCT CC-3’

18S nrDNA: Bult et al. (1992)

N-NS1a 5’-GTA GTC ATA TGC TTG TCT C-3’

N-18Gb 5’-AGG GCA AGT CTG GTG CCA-3’

C-18La 5’-GAA ACC TTG TTA CGA CTT-3’

C-18Hb 5’-GCC CTT CCG TCA ATT CCT TTA AGT TTC AGC-3’

26S nrDNA: Kuzoff et al. (1998)

1Fa 5’-CGA CCC CAG GTC AGG CG-3’

1449Ra 5’-ACT TCC ATG ACC ACC GTC CT-3’

matR: Anderberg et al. (2002)

5’Fa 5’-GTT TTC ACA CCA TCG ACC GAC ATC G-3’

670Fb 5’-TCC TTT GCG CCG CCT TCC TCA TAG AAG-3’

800Rb 5’-AGT ATC GAC CTC CCC GCC CAG CTC TAT CAG C-3’

3’Ra 5’-CGC GGC ACC TGT AGT AGG ACA GAG GA-3’

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a For amplification and sequencing

b For sequencing only


Supplementary Information References

Anderberg, A.A., Rydin, C., Kallersjö, M., 2002. Phylogenetic relationships in the order Ericales s.l.: analyses of molecular data from five genes from the plastid and mitochondrial genomes. Am. J. Bot. 89, 677–687.

Bult, C., Kallersjö, M., Suh, Y., 1992. Amplification and sequencing of 16/18S rDNA from gel-purified total plant DNA. Plant Molec. Biol. Reporter 10, 273–284.

Huang, Y., Shi, S., 2002. Phylogenetics of Lythraceae sensu lato: a preliminary analysis based on chloroplast rbcL gene, psaA-ycf3 spacer, and nuclear rDNA Internal Transcribed Spacer (ITS) sequences. Int. J. Plant Sci. 163, 215–225.

Kuzoff, R.K., Sweere, J.A., Soltis, D.E., Soltis, P.S., Zimmer, E.A., 1998. The phylogenetic potential of entire 26S rDNA sequences in plants. Mol. Biol. Evol. 15, 251–263.

Olmstead, R.G., Bremer, B., Scott, K.M., Palmer, J.D., 1993, A parsimony analysis of the Asteridae sensu lato based on rbcL sequences. Ann. Mo. Bot. Gard. 80, 700–722.


SI Table S3. Areas, time slices, dispersal probabilities, and area connections used in BioGeoBEARS analysis of Myrtales.

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Areas

A = Eurasia, from western Europe, Medit Africa, to temp Asia

B = Africa subSahara, Madagascar

D = Southeast Asia, including India, IndoChina, the Malaysian Peninsula, the Philippines, Sumatra, Borneo and the Inner Banda Arc

E = Australia, including New Guinea, New Caladonia and New Zealand, as well as the Pacific Islands (e.g. Hawaii)

F = North America

G = South America, including most Mexico, S Central America and the West Indies

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Time Slices

0 – 30 Mya

30 – 60 Mya

60 – 90 Mya

90 – 120 Mya

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Dispersal probabilities

0 – 30 Mya

A B D E F G

1 1 1 0.1 1 0.1

1 1 1 0.5 0.1 0.5

1 1 1 1 0.1 0.1

0.1 0.5 1 1 0.1 0.5

1 0.1 0.1 0.1 1 1

0.1 0.5 0.1 0.5 1 1


30 – 60 Mya

A B D E F G

1 1 1 0.1 1 0.1

1 1 1 0.1 0.1 0.1

1 1 1 0.5 0.1 0.1

0.1 0.1 0.5 1 0.1 1

1 0.1 0.1 0.1 1 0.1

0.1 0.1 0.1 1 0.1 1


60 – 90 Mya

A B D E F G

1 0.1 1 0.1 1 0.1

0.1 1 0.1 1 0.1 0.1

1 0.1 1 0.1 0.1 0.1

0.1 1 0.1 1 0.1 1

1 0.1 0.1 0.1 1 1

0.1 0.1 0.1 1 1 1


90 – 120 Mya

A B D E F G

1 0.1 0.1 0.1 1 0.1

0.1 1 0.1 1 0.1 1

0 0 1 0 0 0

0.1 1 0.1 1 0.1 1

1 0.1 0.1 0.1 1 0.1

0.1 1 0.1 1 0.1 1

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Area connections (for all time slices)

A B D E F G

1 1 1 1 1 1

1 1 1 1 1 1

1 1 1 1 1 1

1 1 1 1 1 1

1 1 1 1 1 1

1 1 1 1 1 1



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SI Table S4. Species, designated terminal clade, and proportion of extant species number sampled per terminal in the BAMM analysis of Myrtales.

Species

Terminal

Proportion










Melastoma

Heter

0.0065

Osbeckia

Heter

0.0065

Pterolepis

Heter

0.0065

Tibouchina

Heter

0.0065

Heterocentron

Heter

0.0065

Rhexia

Heter

0.0065

Arthrostemma

Heter

0.0065

Rhynchanthera

Rhync

0.0108

Lavoisiera

Rhync

0.0108

Medinilla

Medi

0.0012

Blakea

Blas

0.006

Bertolonia

Bert

0.0385

Miconia

Clid

0.0025

Tococa

Clid

0.0025

Clidemia

Clid

0.0025

Leandra

Clid

0.0025

Macrocentrum

Graf

0.0138

Graffenrieda

Graf

0.0138

Meriania

Graf

0.0138

Bellucia

Bell

0.0233

Henriettea

Bell

0.0233

Mouriri

Meme

0.0046

Memecylon

Meme

0.0046

Pternandra caerulescens

Pter

0.1333

Pternandra echinata

Pter

0.1333

Olinia

Olin

0.2

Penaea

Pena

0.0435

Rhynchocalyx

Rhyn

1

Alzatea

Alza

0.5

Crypteronia

Cryp

0.1

Eugenia

Myrt

0.0013

Myrtus

Myrt

0.0013

Uromyrtus

Myrt

0.0013

Syzygium

Syzy

0.0008

Tristaniopsis

Kani

0.0192

Lindsayomyrtus

Lind

1

Tristania

Tris

0.1

Metrosideros carminea

Metr

0.0385

Metrosideros macropus

Metr

0.0385

Backhousia

Back

0.1

Callistemon

Mela

0.0056

Melaleuca

Mela

0.0056

Osbornia

Osbo

1

Leptospermum

Lept

0.0138

Kunzea

Lept

0.0138

Chamelaucium

Cham

0.00196

Eucalyptus

Euca

0.0034

Angophora

Euca

0.0034

Stockwellia

Euca

0.0034

Lophostemon

Loph

0.1429

Psiloxylon

Psil

1

Heteropyxis

Hete

0.3333

Ruizterania

Ruit

0.1111

Qualea

Qual

0.0154

Vochysia

Voch

0.0083

Erisma

Eri1

0.0455

Erismadelphus

Eri2

0.3333

Trapa

Trap

0.1667

Sonneratia

Sonn

0.1667

Duabanga

Duab

0.5

Lagerstroemia

Lage

0.0435

Ammania

Amma

0.0122

Lawsonia

Laws

0.0625

Lythrum

Lyth

0.0444

Peplis

Lyth

0.0444

Decodon

Deco

1

Galpinia

Galp

0.1

Punica

Puni

0.25

Cuphea

Cuph

0.0035

Adenaria

Aden

0.2

Heimia

Heim

0.0286

Fuchsia procumbens

Fuch3

1

Fuchsia cyrtandroides

Fuch1

0.3333

Fuchsia paniculata

Fuch2

0.0097

Circaea

Circ

0.125

Hauya

Hauy

0.5

Oenothera

Oeno

0.0048

Clarkia

Clark

0.0189

Epilobium

Epil

0.0058

Gongylocarpus

Gong

0.5

Lopezia

Lope

0.0455

Ludwigia

Ludw

0.011

Terminalia catappa

Term

0.0345

Terminalia kaernbachii

Term

0.0345

Terminalia ivorensis

Term

0.0345

Terminalia phanerophlebia

Term

0.0345

Terminalia ulexoides

Term

0.0345

Terminalia amazonia

Term

0.0345

Terminalia bucidoides

Term

0.0345

Buchenavia

Term

0.0345

Conocarpus

Cono

0.5

Bucida

Bucid

0.3333

Combretum collina

Comb

0.01838

Combretum imberbe

Comb

0.01838

Quisqualis

Comb

0.01838

Combretum farinosum

Comb

0.01838

Combretum rotundifolium

Comb

0.01838

Macropteranthes

Macro

0.2

Lumnitzera

Lumn

0.5

Laguncularia

Lagu

1

Strephonema mannii

Strep

0.6667

Strephonema pseudocola

Strep

0.6667

Xylonagra

Xylo

1

Xanthostemon

Xant

0.02

Syncarpia

Sync

0.5

Astronia

Astr

0.006

Merianthera

Camb

0.033

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