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Figure 1A. Absorbance vs. concentration for spectrophotometric determination



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TUTORIAL ORIGIN S09

Figure 1A. Absorbance vs. concentration for spectrophotometric determination 
of ferrous iron
c. Set the font size to 24 and change the color to black. 
d. Click 
OK”. 
NOTES:
1. The text will likely run over the side of the figure. Decide on a place or places to split the title; 
double click at any point on the text; place the cursor at the point in the text box at which the text is to be 
split; press the “Enter” key on the keyboard and click on “OK”. 
2. Text can be moved either by dragging it with the left mouse button held down or by clicking on it 
and using the “up/down”, “left/right” arrows on the keyboard. 
e. Use the “Text” procedure to place an upper-case A in the upper left-hand corner of your 
figure and to label the figure in the lower left hand corner of the page as follows: 
DISK: CHEM XXX ; FILE: ORIG1_A; PLOT1, COLUMNS A AND B in which XXX is your course number. 
J. Printing a figure 
a. Make sure the figure is in the forefront of the screen. 
b. To format your figure for printing, Click on “File/Page setup/ Portrait/OK”. 
c. To print your figure, click on “File/Print/OK”. 
SAVE YOUR FILE ON A FLOPPY DISK AS ORIG1_A.ORG BEFORE PROCEEDING WITH THE NEXT 
PART. 
K. Preparing a template 
This section explains how you can prepare and save a template that will help you avoid having to 
repeat some of the steps above each time you prepare a new figure. 
a. Bring the worksheet to the forefront and double click on the green button in the upper left 
corner and select delete from the menu. The worksheet will disappear and the data will 
disappear from your figure. 
b. Click 
File/New/Worksheet/OK”; a new worksheet will appear. 
c. Use 
Save As” to save this template as “Formatted Template” on your disk.
d. To illustrate the use of the template, Click File/Close. Then Click “File/Open/A/FORMATTED 
TEMPLATE”. Click “File/New/Worksheet/OK”; a new worksheet will appear. Enter the 
numbers 2, 4, 6, 8 in rows 1 – 4 of Columns A and B of the worksheet. Then highlight data in 
column B, bring the blank figure to the forefront, Click “Graph/Add Plot to Layer/Line + 
Symbol”; a Line + symbol plot should appear. 
e. Close this file without saving changes before proceeding to PART III.
Note: You can use the FORMATTED TEMPLATE for any of the plots you will do in the remainder of this 
tutorial or this course. It will usually be necessary to ranges of axes, axis labels, titles, etc., but the 
template should save you significant amounts of time.


6

III. LEAST-SQUARES FIT OF A LINEAR MODEL
The Origin software includes built-in least-squares fitting routines for several types of functions
including linear relationships such as that represented by the absorbance vs. concentration data plotted 
above. The purpose of this section is to illustrate the use of the linear fitting function and subsequent 
plotting of results. 
A. Rationale 
The absorbance vs. concentration data are expected to follow a linear relationship of the form 
A
a bC
= +
(1a) 
in which A is absorbance, a and b are the intercept and slope, respectively, and C is concentration. This 
expression can be written in the more general form 
Y
a bX
= +
(1b) 
Either way, the goal of a fitting process is to obtain best-fit values of the intercept, a, and slope, b as well 
as other statistical quantities to be discussed in class. 
NOTE: Use a different file name, e.g. ORIG1_B, to save modifications made to this plot in subsequent 
steps. 
B. Fitting process - built-in algorithm 
There are different ways to use Origin to do a least-squares fit of a linear model to a data set. One of 
the simpler options is illustrated here.
Numerical values of slopes, intercepts etc. will be displayed on the screen below the “plotting 

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