Organizing co mmittee

 
 

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Poster presentation 
342 
CHEMICAL COMPOSITION OF HEXANE EXTRACT OF 
CALLUS CULTURE AND REGENERANTS OF THE MEDICINAL 
PLANT NIEDZWEDZKIA SEMIRETSCHENSKIA
Sarsenbek B.T.
1
, Bobakulov Kh.M.
2
, Sanoev A.I.
2
, Mursaliyeva V.K.
3
,
Turasheva S.K.
1
, Sagdullayev Sh.Sh.
2 
 
1
Al-Farabi Kazakh National University, Almaty, Republic of Kazakhstan, 
e-mail: 
[e-mail]
, Tel. [phone] 
2
Institute of the Chemistry of Plant Substances named after academician S. Yu. Yunusov 
of the Academy of Sciences of Uzbekistan, Tashkent, Republic of Uzbekistan 
3
Institute of plant biology and biotechnology, Almaty, Republic of Kazakhstan 
Medicinal plant 
N. semiretshenskia
B. Fedtsch 
(Incarvillea niedzwedzkia)
is a 
representative of the 
Bignoniaceae
family and belongs to a relict endemic species listed 
in the Red Book of Kazakhstan and the Red Book of the International Union for 
Conservation of Nature and Natural Resources.
 
Chemical studies have shown that species of the genus 
Incarvillea
are rich sources of 
secondary metabolites with anti-inflammatory and antinociceptive activity. In this 
regard, volatile compounds of the species 
N. semiretschenskia
have not been studied in 
natural conditions and in culture. 
The purpose of this work is to study the chemical composition of hexane extract of 
this species cultivated 
in vitro
. 
Hexane extract from callus and regenerant plants of 
N. semiretschenskia
was 
obtained at a temperature of 20°C for 4-5 hours. The resulting extract was filtered over 
anhydrous Na
2
SO
4
and stored at 4°C in a tightly closed vial before analysis. The 
obtained 
N. semiretschenskia
oils are a pale yellow mobile liquid with a specific odor 
(the yield from the callus is 0.82%, from regenerate plants 0.74%). 
The obtained extracts were analyzed on an Agilent 7890A GC gas chromatograph 
with an Agilent 5975C inert MSD quadrupole mass spectrometer as a detector. The 
components of the mixture were separated on a quartz capillary column HP-InnoWax 
(30 m × 250 µm × 0.25 µm). The volume of the introduced sample is 1 µl (hexane), the 
flow rate of the mobile phase was 1.0 ml/min. The components were identified based on 
a comparison of the characteristics of the mass spectra with data from the electronic 
libraries W8N05ST.L and NIST08. 
As a result of the analysis of hexane extract from regenerating plants, 16 compounds 
were identified, of which the major compounds are nonacosane (24.5%), dodecane 
(22.8%), clionasterol (10.4%), triacontane (9.3%), heptadecane (7.6%), undecane 
(6.3%), palmitic acid (2.9%), linoleic acid (2.9%), ethyl 9,12,15-octadecatrienoate 
(2.4%), heptacosane (1.4%), amounting to 90.5%. 18 compounds were identified from 
callus, such as: 9,12-octadecadienoic acid (17.8%), hexadecanoic acid (10.9%), β-
sitosterol (26.4%), cis, cis, cis-7,10,13-hexadecatrienal (9.4%), 5,12-naphthacenedione 
(6.8%), squalene (6.8%), hexatriacontane (3.5%), octadecane (3.4%), eicosane (3.1%), 
hexadecane (1.6%), hexacosane (1.5%), amounting to 91.2%. 
Further phytochemical studies of extracts from callus biomass in dynamics and 
comparative chemical analysis of test tube regenerants and plants grown in a 
greenhouse are planned. 

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Poster presentation 
343