Organizing co mmittee

C
OH
CHOH
CH
C
O
HO
CH
3
CH
3
CH
3

---

Poster presentation 
87 
BIOLOGICAL ACTIVITIES OF ALKALOIDS FROM
Rindera oblongifolia 
 
R.M. Ruzibaeva, P.A. Nurmakhmadova, R.P. Zakirova,
N.I. Mukarramov, A.M. Nigmatullaev 
 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: [e-mail] 
 
Currently, extracts from plant materials are widely used in the pharmaceutical, food 
industry and agriculture. However, use of chemicals give good results, in agriculture it 
harms the environment and significantly worsens the quality of vegetable crops. Natural 
ingredients. a will modern and safe alternative be biological growth stimulants. They 
don’t just improve vegetation, but also make plants more adaptable to environmental 
conditions. For this reason, the purpose of this work is to study the biological activity of 
hydroalcoholic extracts obtained from plants - 
Rindera oblongifolia 
M.Popov. 
Rindera oblongifolia
M. Popov collected from the Pskom mountains of Tashkent 
region, wich was extracted with 80% ethanol using an ultrasonic extractor [1]. The 
alcohol was evaporated by rotary evaporator, the aqueous part was mixed to an acidic 
medium (pH=2-3) with a solution of sulfuric acid, non-polar substances were extracted 
with chloroform. The remaining aqueous part was blended wich alkaline medium 
(pH=8-9) in 10% ammonia solution and also extracted with chloroform. The chloroform 
was removed and the aqueous fraction was extracted with ethyl acetate to get an ethyl 
acetate fraction. Echinatin and trachelanthamine N-oxide were isolated from the 
chloroform part, and new alkaloids rinderidine and oblongifolidin were obtained from 
the ethyl acetate part [2]. 
Next, tests were carried out the growth-promoting activity of the alkaloids 
concentrations were tested in the range of 0.1% - 0.00001%. 
The results of the primary screening, echinatin and oblongifolidin showed growth-
stimulating activity to the wheat seeds at 0.00001% concentrations. The growth of the 
root of plant of wheat was 8.8 sm and 8.1 sm, which exceeded the control (water) 
variant by 37.5% and 26.5%, respectively. The growth of wheat shoots was 5.6 sm and 
5.1 sm, where percentage terms it was 40.0% and 27.5% more than the control (water). 
References: 
1. Vvedenskiy A.I.1961. Flora of Uzbekistan, Vol. 5, Tashkent:Publ.Acad.Nauk 
UzSSR; pp 196-202.Russian 
2. R.M. Ruzibaeva, Kh. M. Bobakulov, N. I. Mukarramov, B. Tashkhodzhaev, R. 
Ya. Okmanov, A. M. Nigmatullaev & N. D. Abdullaev “Rinderidine and 
oblongifolidine new pyrrolizidine alkaloids from 
Rindera oblongifolia 
M. Popov and 
their 
absolute 
configurations“. 
Natural 
Product 
Research, 
DOI: 
10.1080/14786419.2022.2134865 

---

Poster presentation 
88