Isolation of chemical constituents towards standardization



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ISOLATION OF CHEMICAL CONSTITUENTS

TOWARDS STANDARDIZATION

OF

Syzygium cumini seeds

SYNOPSIS FOR
M.PHARM DISSERTATION
SUBMITTED TO

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

BY
Patel Salim Kasam
Department of Pharmacognosy
THE OXFORD COLLEGE OF PHARMACY
BANGALORE-78


RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,
KARNATAKA, BANGALORE
ANNEXURE-II
PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

1.

Name of the candidate and Address

Patel Salim Kasam

The Oxford College of Pharmacy,

J.P. Nagar, Ist Phase,

Bangalore-560078.
PERMANENT ADDRESS:

S/o Kasam Karim Patel,

near borker vasti, post-Kolpewadi,

Tal- Kopergaon

Dist-Ahmednagar

Pin- 423602


2.

Name of the Institution

The Oxford College of Pharmacy, J.P.Nagar, Ist Phase,

Bangalore-560078.


3.

Course of study and subject

M.Pharm, Pharmacognosy



4.

Date of Admission

6th June 2007



5.

Title of the Topic

ISOLATION OF CHEMICAL CONSTITUENTS TOWARDS STANDARDIZATION OF Syzygium cumini seeds







6.




Brief resume of the intended work:
6.1- Need for the study:
Syzygium cumini is also called as jamun, belongs to the family Myrtaceae.

Syzygium cumini contains lignan glucosides such as medioresinol 4"-O-β-glucoside1, monoterenes and sesquiterpenes i.e. pinocarveol, α-terpineol, myrtenol, eucarvone, muurolol, myrtenal, α-cadinol and pinocarvone2, fatty acids i.e. lauric, myristic,palmitic, stearic, oleic, andc vernolic acid3 and volatile components i.e. ocimene and β-myrcene4.

Different extracts of Syzygium cumini were evaluated for antibacterial5, anticonvulsant, sedative6, hypoglycaemic7, antiallergic8, hepatoprotective9 and gastroprotective activity10. The inhibitory effect of syzygium cumini bark is shown on the inflammation induced by autocoids11.

Although more emphasis has been given to the various aspects of the plant, very less importance has been given towards the isolation of chemical constituents and standardization of Syzygium cumini seeds.

Therefore, in the present study it is proposed to isolate the chemical constituents from seeds of Syzygium cumini and characterize them for standardization.



6.2 – Review of Literature:


  1. Lignan glucosides such as medioresinol 4"-O-β-glucoside, pinoresinol-O-β-glucoside was reported from methanolic extract of the seeds of Syzygium cumini1.

  2. Pinocarveol, α-terpineol, eucarvone and pinocarvone was reported from the fresh leaves of Syzygium cumini2.

  3. Fatty acid was reported from the seed oil of Syzygium cumini3.

  4. Volatile component, ocimene and β-myrcene was reported as major compounds from Syzygium cumini fruits4.

  5. Antibacterial activity was reported from ethanolic extract of Syzygium cumini5.

  6. Sedative and anti-convulsant activities was reported from different extracts of Syzygium cumini seeds6.

  7. Hypoglycaemic activity was reported from aqueous extract of Syzygium cumini seeds7.

  8. Antiallergic activity was reported from aqueous leaf extract of Syzygium cumini8.

  9. Hepatoprotective activity was reported from the aqueous leaf extract of Syzygium cumini9.

10.Gastro-protective effect of tannins was reported from Syzygium cumini10.

11.Anti-inflammatory effect was reported from the ethanolic extract of Syzygium cumini

bark11.

7.



6.3 - Objective of the Study:


  • Collection of the seeds of Syzygium cumini from Natural Remedies Pvt. Ltd.




  • Preparation of the extract of the drug.




  • Isolation of chemical constituents from the extract by column chromatography or preparative HPLC.







  • Development of chromatographic fingerprint for the isolated compounds as markers using HPLC/HPTLC.



Materials and Methods:

7.1 a) Source of Data:


Search on Medline and other Journals from The Oxford College of

Pharmacy, Govt. College of Pharmacy, RGUHS-Digital library.


b) Place of work: Natural Remedies Pvt.Ltd, and The Oxford College of

Pharmacy.

7.2 Method of Collection of Data:




  • Collection - Authenticated samples of seeds of Syzygium cumini will be collected from Natural Remedies Pvt. Ltd. Bangalore.

  • Extraction- Extracts of the drugs will be prepared using different solvents by maceration/ reflux.

  • Isolation- Isolation of phyto-constituents will be done using column chromatography or preparative HPLC.

  • Identification and characterization- The isolated compounds will be identified using advanced analytical methods like NMR, IR and Mass spectroscopy.

7.3 - Does the study require any investigations or inventions to be conducted on

patients or other humans or animals?

If so, please describe briefly.

- Not applicable -

7.4 - Has ethical clearance been obtained from your institution in case of 7.3?


- Not applicable -








8.



Bibliography:


  1. Martin TS, Ohtani K, Kasai R, Yamasaki K. Lignan glucoside from Syzygium cumini. Natural Medicines 1998;52(4):360-363.

  2. Jirovetz I, Buchbauer G, Puschmann C, Fleischhacker W, Shafi PM, Rosamma MK. Analysis of the essential oils of the fresh leaves of Syzygium cumini and Syzygium travancoricum from South-India. Journal of Essential Oil Bearing Plants 1999;2(2):68-77.

  3. Mahmood JD, Mirajkar AM, Hosmani KM, Mulla GMM. Epoxy and cyclopropenoid fatty acids in Syzygium cumini seed oil. Journal of Science in Food and Agriculture 1988;43(1):91.

  4. Vijayanand P, Jagan Mohan Rao L, Narasimham P. Volatile flavour components of jamun fruit (Syzygium cumini L). Flavour Frag J 2001;16:47-49.

  5. Chattopadhyay D, Sinha BK, Vaid LK. Antibacterial activity of Syzygium species. Fitoterapia 1998;69(4):365-367.

  6. De lima TCM, Klueger PA, Pereira PA, Macedo-Neto WP, Morato GS, Farias MR. Behavioural effects of crude and semi-purified extracts of Syzygium cumini Linn. Skeels. Phytotherapy Research 1998;12(7):488-493.

  7. Prince PSM, Menon VP, Pari L. Hypoglycaemic activity of Syzygium cumini seeds: effect on lipid peroxidation in alloxan diabetic rats. Journal of Ethnopharmacology 1998;61(1):1-7.

  8. Britol FA, Lima LA, Ramos MFS, Nakamura MJ, Cavalher SC-Machado, Siani AC, et al. Pharmacological study of anti-allergic activity of Syzygium cumini(L.) Skeels. Braz J Med Bio Res 2007;40(1):105-15.

  9. Rafael NM, Rita LS, Anie SB, Ricardo FC, Patricia G. Effect of the aqueous extract of Syzygium cumini on carbon tetrachloride induced hepatotoxicity in rats. Phytother Res 2007;21(8):793-5.

  10. Rabecca OR, Camilo CR. The gastroprotective effect of tannins extracted from duhat(Syzygium cumini Skeels) bark on HCL/ethanol induced gastric mucosal injury in Sprague Dawley rats. Clinical Hemorheology and Microcirculation 2003;29(3-4):253-261.


  1. Murugnandan S, Pant S, Srinivasan K, Chandra S, Tandan SK, Lal J, et al. Inhibitory role of Syzygium cumini on autocoid-induced inflammation in rats. Indian J Physiol Pharmacol 2002;46(4):482-6.






9.

Signature of Candidate



10.

Remarks of the Guide


Recommended



11.

11.1 Name and Designation of Guide

Dr. Padmaa M. Paarakh

Professor and Head

Department of Pharmacognosy





11.2 Signature










11.3 Co-Guide



Dr. Deepak. M

Manager – Phytochemistry (R&D)

Natural Remedies Pvt. Ltd.

Bangalore - 560100




11.4 Signature








11.5 Head of the Department


Dr. Padmaa M. Paarakh

Professor and Head

Department of Pharmacognosy





11.6 Signature






12.

12.1 Remarks of the Chairman and

Principal


Forwarded to the University for scrutiny






12.2 Signature


Dr. Padmaa M. Paarakh

Principal

The Oxford College of Pharmacy,



J.P.Nagar, Ist Phase, Bangalore – 78.


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