Nitrogen Estimation by Kjeldahl’s Method



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SUMMARY

India have rich heritage of herbal drugs. Some of these are subjected to systematic cultivation in the specific areas. In these areas many medicinal plants are naturally occurring but are now in endangered and in future these plants would have been extinct or no more in the area.

Medicinal Plants collected from the part of Nagnath ,Buttenath and Parli ghat are cultivated in the vicinity of college campus.

After a year some medicinal plants are used to plant in the area of Nagnath and Buttenath for increasing the population in this area.

After the complete growth of medicinal plants, harvesting has done for standards drugs in this plant parts are dried in natural condition for removal of moisture content of crude drug .This practice maintain the natural colour of the drug and volatile principle present in it. Plant material selected for medicinal value ground into fine powder with the help of grinder.

Nitrogen Estimation by Kjeldahl’s Method

Micro Kjeldahl’s method is suitable method for the estimaton of total nitrogen content of medicinal plant material, food material, clinical samples and agricultural products.

Kjeldahl’s method is based on the fact that when an organic compound contain nitrogen was heated with conc.H2SO4 the nitrogen in it was converted into ammonium sulphate. The resultant liquid is then treated with excess alkali and liberated ammonia gas absorbed in the excess of standard acid. The nitrogen is determined by finding the amount of acid neutralized by blank titration with standard alkali.

Detail procedure of nitrogen estimation and calculation included in the hard bound copy.

Leaves of Adhatoda vasica contain 5.83% of nitrogen, Syzygium cumini 4.2% of nitrogen,Vitex nigundo 1.86%, Tylophora indica 5.1% ,Gloriosa superba contain 4.9%, Afumari 5.13%, Aloe vera 5.3% ,Tinospora condifolia 1.8% ,Anacyclus pyrethrum 4.5% ,Vitis quadrangularis stem contain 3.5%, Solanum anguivi contain 3.7% of nitrogen .

Spectroscopic studies of medicinal plants

The spectrophotometer is useful for measuring the absorption spectrum of a compound. The spectroscopic techniques are generally employed to measure the energy difference between various molecular energy levels.

Procedure of spectroscopic studies of some medicinal plant mentioned in the hard bound copy and results are plotted in the form of graph .

Leaf sample of Aloe vera having maximum absorption spectra 436nm ,absorption peak of Adhatoda vasica was measure at 660nm ,sample of Tylophora indica was shown 363nm ,Tinospora condifolia sample having many peaks ranging 208 nm-379nm .This peak shows presence of various active compound in the sample.

Sample of Anacyclus pyrethrum having absorption spectra which was ranging from 319-359nm ,leaves of Syzygium cumini showed 786 nm spectra on UV spectrophotometer, stem of Gymnema sylvestre shows bands of 361nm on UV spectrophotometer.

In the stem sample of Solanum anguivi 336nm max was observed.Sample of Vitex nigundo observed under UV spectrophotometer was obtained 364 nm max.



Study of some alkaloid and effect of medicinal plant

material on oraganism

Various alkaloid are reported from leaves,seed,roots,stem and bark of medicinal plants.

Leaves of Aloe barbadenses contain barbodos, leaves of Adhatoda vasica contain vasicine.Medicinal plant extract was use to feed to rat found healthy growth of rat.So this extra contain carbohydrates ,alkaloid ,vitamin ,protein and growth regulator.

The extract of Adhatoda vasica leave was used to measure activity against Staphylococcus and E. coli observed that ,this plant material act as growth inhibitor to bacteria. The anti-bacterial activity of Tinospora cardifolia extracts has been assayed against E.coli, staphylococcus and Salmonella sp.



Uses of some medicinal plants are included in the hard bound copy.


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