Ascorbate-glutathione cycle for scavenging H
2
O
2
in bread wheat (Triticum aestivum L.) genotypes
63
Husbandry, Azerbaijan
Republic Ministry of
Agriculture. Plants were grown under field
conditions (+25°C, 72% humidity) and the
activities of APX,
GR and the amount of H
2
O
2
,
AsA, GSH were determined during drought and
rehydration in the wax ripening phase of
ontogenesis. Leaf samples
from drought-exposed
and rewatered (after 7 days of rehydration) plants
were taken during the wax ripening phase of
ontogenesis and frozen in liquid nitrogen. The
experiments continued under laboratory conditions.
Hydrogen peroxide determination: The amount
of hydrogen peroxide
was determined by the
spectrophotometric
method
of
Bellinkampi
(Bellincampi et al., 2000). This method is based on
the reduction of Fe
+2
ions to Fe
+3
ions by xylene
orange. Leaf samples of 0.2-0.3 g were taken from 3
variants
of plants - control, drought-exposed, and
rehydrated plants. The leaves were ground in liquid
nitrogen and 1-1.5ml of cold 100% acetone was
added
and after thorough mixing, it was centrifuged
at 12, 000 g for 10 min. Then 0.5 ml of the obtained
supernatant was added to the same amount of xylene
orange. Samples were stored at room temperature for
45 min and stirred periodically. After completion of
the reaction, the mixture was centrifuged again for 5
min, at 10,000g. Optical density was determined at
560 nm in Thermo Scientific Evolution 350 UV-Vis
Spectrophotometer. Standards
were prepared using
30% H
2
O
2
.
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