Organizing co mmittee

ANTIFUNGAL ACTIVITY OF ENDOPHYTIC BACTERIA
FROM SOYBEAN 
M.Z. Alimova
1
, V.V. Shurigin
2 
 
1) National University of Uzbekistan, Tashkent, 100174, Uzbekistan 
2) Institute of Microbiology of the ASc RUz, Tashkent, 100128, Uzbekistan 
 
Plant diseases are one of the major barriers to high yields, but they can potentially be 
biologically controlled with PGPR and endophytic bacteria. Some of the mechanisms 
that PGPR and endophytic bacteria use to counteract the harmful effects of 
phytopathogens include: production of fungal cell walls degrading enzymes such as 
lipase, β-1,3-glucanase, chitinase, and protease; synthesis of hydrogen cyanide, which 
inhibits cytochrome C oxidase, as well as other important metalloenzymes; synthesis of 
siderophores, which can prevent or reduce the proliferation of pathogens by reducing 
the amount of iron available to pathogens, etc. 
The aim of our research was to isolate endophytic bacteria from soybean (
Glycine 
max
), screen them for antifungal activity, and study the biocontrol properties of isolated 
bacteria. 
In total 20 isolates (SOEN-1 - SOEN-20) of endophytic bacteria were isolated from 
the roots and stems of soybean. The isolated isolates were tested for their ability to 
inhibit the growth of phytopathogenic fungi 
Fusarium oxysporum
and 
Fusarium solani
(Figure). The isolates SOEN-2, SOEN-9, SOEN-11, SOEN-15, and SOEN-20 inhibited 
phytopathogenic fungi more effectively than other bacterial isolates and therefore were 
selected for further study. 
Fig. Growth inhibition of phytopathogenic fungi by endophytic bacteria from soybean 
(on Petri dishes) 
Selected bacteria were tested for their antifungal properties. It was revealed that all 5 
isolates had certain antifungal properties. The isolate SOEN-2, which previously 
showed the highest antifungal activity, was able to produce lipase, protease, glucanase, 
chitinase, and HCN. The isolate SOEN-9 produced lipase, protease, chitinase, and 
siderophores, while SOEN-15 produced protease, glucanase, chitinase, and HCN. The 
isolates SOEN-11 and SOEN-20 had only 3 out of 6 tested antifungal properties. 

---

Poster presentation 
201