Organizing co mmittee

 
 

---

Poster presentation 
314 
THE PRODUCERS FOR MICROBIAL INOCULANT IMPROVING 
PLANT GROWTH IN SALINE CONDITIONS 
M.Z. Isoqulov
1
, V.V. Shurigin
2 
 
1) National University of Uzbekistan, Tashkent, 100174, Uzbekistan 
2) Institute of Microbiology of the ASc RUz, Tashkent, 100128, Uzbekistan 
In recent years, soil salinization has become one of the most acute global problems of 
agriculture around the world. Salinization contributes to the rapid degradation of 
agricultural land. The current situation requires scientists to develop effective measures 
to prevent this problem. One of the effective approaches to solve the problem is the 
development of an effective biotechnology for obtaining biological preparations to 
improve the quality of plants and increase their yield.
The aim of our research was to isolate salt-tolerant bacteria from the rhizosphere of 
cotton and wheat and to select the most effective bacteria that stimulate the growth of 
tomato and cucumber under soil salinity conditions. 
In total we isolated 5 isolates of 
Azotobacter
spp., 8 isolates of 
Bacillus
spp. and 8 
isolates of 
Pseudmonas
spp. from the rhizosphere of wheat and cotton grown in saline 
soils. As a result of screening of bacterial isolates for activity in stimulating the growth 
of cucumber and tomato under saline conditions, the most active isolates 
Azotobacter
sp.-2, 
Azotobacter
sp.-5, 
Pseudomonas
sp.-1, 
Pseudomonas
sp.-7, 
Bacillus
sp. -3 and 
Bacillus
sp.-6 were selected. 
To test the effect of various selected strains of 
Azotobacter
sp.-2, 
Azotobacter
sp.-5, 
Pseudomonas
sp.-1, 
Pseudomonas
sp.-7, 
Bacillus
sp.-3 and 
Bacillus
sp.-6 on the growth 
and yield of cucumber and tomato, the following experiment was carried out. The plants 
were growing in large containers with saline and non-saline soil. At the same time, 
seeds were inoculated and the soil was fertilized with a bacterial suspension of various 
combinations of bacterial strains. The mixture of 
Azotobacter
sp.-5, 
Pseudomonas
sp.-1 
and 
Bacillus
sp.-3 strains proved to be the most effective. So, when growing cucumber 
of the Navruz variety using this mixture of bacterial strains, the stem length increased 
by 35% in non-saline soil and by 32% in saline soil, the root length in non-saline soil 
increased by 70%, and in saline soil by 47%, fruit weight increased by 6.5 g in saline 
soil and 7.3 g in non-saline soil, the number of fruits increased from 3 in control to 7 
when inoculated in saline soil and from 5 to 10 in non-saline soil. 
In the case of tomato, the mixture of 
Azotobacter
sp.-5, 
Pseudomonas
sp.-1 and 
Bacillus
sp.-3 strains also proved to be the most effective. So, as a result of the use of an 
inoculum based on these strains, the length of the stem of the Istiklol variety increased 
by 24% in non-saline and 20% in saline soil, the length of the main root increased by 
52% in non-saline and 44% in saline soil, fruit weight increased by 7 g in saline and 
10.9 g in non-saline soil, the number of fruits per plant increased from 3 in control to 6 
after the application of bacterial suspension in saline soil and from 5 to 9 in non-saline 
soil.
In the future research, we plan to conduct field trials of a microbial inoculant based 
on a mixture of 
Azotobacter
sp.-5, 
Pseudomonas
sp.-1, and 
Bacillus
sp.-3 strains. In the 
case of positive results, this microbial association can be used to obtain a microbial 
preparation increasing plant yields under saline conditions. 

---

Poster presentation 
315