Appendix A. Supplementary material for online version SI Figure S1. Bayesian primary concordance trees of Myrtales using BUCKy. See Fig. 1 for comparison.
SI Figure S2. Ancestral range estimation (ARE) on the Myrtales chronogram with BioGeoBEARS (DEC+J model). Output shows the frequency (as a proportion of the pie) of each likely area (or combined area) at each node and corner. See Fig. 3 that shows only the dominant area (or combined area) at each node and corner.
SI Table S1. Species, voucher/collection information, and GenBank accession numbers.
SI Table S2. Primers used for amplification and sequencing of rbcL, matK, ndhF, 18S nrDNA, 26S nrDNA and matR.
Primer Sequence Reference
rbcL: Huang and Shi (2002)
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Bult, C., Kallersjö, M., Suh, Y., 1992. Amplification and sequencing of 16/18S rDNA from gel-purified total plant DNA. Plant Molec. Biol. Reporter 10, 273–284.
Huang, Y., Shi, S., 2002. Phylogenetics of Lythraceae sensu lato: a preliminary analysis based on chloroplast rbcL gene, psaA-ycf3 spacer, and nuclear rDNA Internal Transcribed Spacer (ITS) sequences. Int. J. Plant Sci. 163, 215–225.
Kuzoff, R.K., Sweere, J.A., Soltis, D.E., Soltis, P.S., Zimmer, E.A., 1998. The phylogenetic potential of entire 26S rDNA sequences in plants. Mol. Biol. Evol. 15, 251–263.
Olmstead, R.G., Bremer, B., Scott, K.M., Palmer, J.D., 1993, A parsimony analysis of the Asteridae sensu lato based on rbcL sequences. Ann. Mo. Bot. Gard. 80, 700–722.
SI Table S3. Areas, time slices, dispersal probabilities, and area connections used in BioGeoBEARS analysis of Myrtales.
A = Eurasia, from western Europe, Medit Africa, to temp Asia