www.ijps.info Int. J. Ph. Sci. | Sept-December 2009 | Vol. 1 | Issue 2 |330-332
ANTIHYPERLIPIDEMIC ACITIVITY OF SYZYGIUM CUMINI LINN. SEED
EXTRACT ON HIGH CHOLESTEROL FED DIET RATS
MODI DIKSHIT C
, RACHH PR
, NAYAK BS
, SHAH BN
, MODI KP
, PATEL NM
1. Vidyabharati Trust College of Pharmacy, Umrakh, Bardoli, Surat, 394345;
2. Shri B.M. Shah College of Pharmaceutical Education and Research, Modasa, Gujarat, India, 383315.
3. Nootan Pharmacy College, Visnagar.
* For Correspondence: Mr. Dixit C. Modi, email@example.com
Coronary arterial diseases are responsible for more
deaths than all other associated causes combined.
Elevated serum cholesterol levels leading to
atherosclerosis can cause coronary heart disease
. Hyperlipidemia is a major cause of
conditions, such as coronary heart disease (CHD),
ischemic cerebrovascular disease, and peripheral
. Among these hypercholesterolemia
ischemic heart disease
. Reduction in serum
. Currently available hypolipidemic
effects. Medicinal plants play a major role in
. The plant Syzygium
India where it is used as a antiasthmatic,
antidiarrhoeal, and anti-diabetic remedy. Current
investigation was to study effect of alcoholic extract
induced hyperlipidemia in rats.
Cholesterol, sodium cholate and coconut oil were all
purchased from SD-fine chemicals, India., All other
reagent used was of analytical grade.
Centrifuge Machine (Eltek-research centrifuge-TC-
The seeds of Syzygium cumini Linn. were collected
from the college campus of Modasa-Gujarat (India)
and were authetified by Dr. M.S.Jangid, Botany
Department from Sir P.T Science College-Modasa,
India. The seeds were dried, grined and the powder
was used for the extraction.
Extraction of Plant Material
The fruits of Syzygium cumini Linn. (Jamun) were
washed well and pulp was removed. The seeds were
washed with distilled water to remove the traces of
pulp from the seeds and were dried at room
temperature. The seeds were powdered in an
electrical grinder and stored until further use. The
powdered seeds were extracted in a sohxlet with 95%
ethanol at a temperature of 50
Aim: Preliminary phytochemical studies and Antihyperlipidemic activity of
(2% cholesterol, 1% sodium cholate and 2% coconut oil) for seven days in
standard rat chow diet. The Alcoholic extract of Syzygium cumini seeds (100
mg/Kg body weight) was orally administered once a day to rats fed with a high
cholesterol diet for seven days. Results and Discussion: High cholesterol fed
diet rats exhibited significant increase in serum cholesterol, triglycerides, low
density lipoproteins, very low density lipoprotein, atherogenic index and
significant decrease in high density lipoproteins, high density lipoproteins
ratio. Treatment with extract significantly decreased serum cholesterol,
triglycerides, low density lipoproteins, very low density lipoprotein,
atherogenic index and significantly increased the high density lipoproteins,
high density lipoproteins ratio in hyperlipidemic rats. Conclusion:
Antihyperlipidemic activity of Alcholic extract of Syzygium cumini Linn.
which may be due to the presence of alkaloids, Flavonoids, Phenols, Saponins,
Tannis (Gallic acid, Ellagic acid) and Triterpenoids found in the preliminary
resultant extract was filtered. The filtered extract was
temperature of 40
refrigerator and considered as the extract
Preliminary phytochemical screening of the Jamun
seed extract was carried out for the detection of the
various plant constituents
Sprague Dawely female rats weighing 200-250 gm
were acclimatized to the experimental room having
conditions, and 12:12 hour light and dark cycle.
Animals were caged in polypropylene cages in a
group with maximum of three animals per cage. The
rats were fed with standard food pellets and water ad
libitum. The study was approved by Institutional
Animal Ethical Committee, Shri B. M. Shah College
of Pharmaceutical Education and Research, Modasa,
Gujarat, India (IAEC/BMCPER/02/2008-09).
High Cholesterol diet was prepared by mixing
cholesterol 2%, sodium cholate 1% and coconut oil
2%, with standard powdered standard animal food.
The diet was placed in the cage carefully and was
aministered for seven days.
The extract of Jamun seed was dissolved in distilled
water and a dose of 100 mg/Kg was given to the
animals once in a day along with the High
Cholesterol diet orally. Treatment was given daily
for seven days.
The experimental animals were divided into three
groups, six animals in each group
High cholesterol diet treated with
Jamun Extract [100 mg/kg body
weight (b.w.), Orally (p.o.)]
Blood sample collection and analysis
On the 8
day, blood was collected by retro-orbital
hr fasting and allowed to clot for 30 minutes at room
temperature. Blood samples were centrifuged at 3000
rpm for 20 minutes. Serum was separated and stored
C until biochemical estimations were carried
spectrophotometrically for Cholesterol, triglyceride
and HDL-C was estimated using diagnostic kits
which were procured from Lab-Care Diagnostics
(India) Pvt. Ltd.- Mumbai (India).
VLDL, LDL, HDL-ratio and Atherogenic index were
Experimental results were mean
SEM of 6 animals.
various groups were evaluated using one-way
analysis of variance (ANOVA) followed by Tukey
test. Data were considered statistically significant
only when p value < 0.05.
Result and Discussion
The rats fed with High cholesterol diet for seven days
exhibited significant increase in Serum cholesterol,
Triglyceride, LDL, VLDL, Atherogenic index and
significant decrease in HDL and HDL ratio (Table
no. 1). Effect of one week treatment with Jamun
Extract at a dose of 100 mg/kg b.w., p.o.
significantly reduced the elevated serum cholesterol,
LDL, Atherogenic Index and significantly increased
the HDL-C, HDL-ratio. The Gymnema extract had
significant effect on Triglyceride as well has VLDL.
This finding proves that the seed extract of Jamun
can be used for the treatment of Hyperlipidemia.
Hence, apart from the wide usage of Jamun seed as
antidiabetic it can also be used for the treatment of
The Jamun extract at a dose of 100 mg/kg body
weight orally showed Antihyperlipidemic activity
which may be due to presence of Flavonoids,
Phenols, alkaloids, Tannis (Phenolic compounds) and
phytochemical screening. Further studies are needed
to evaluate the antihyperlipidemic potential of the
Jamun seed in clinical study.
Table 1: Effect of Syzygium cumini seed extract on
various parameters in High cholesterol diet rats.
High cholesterol diet
treated with Jamun
Extract (100 mg/kg b.w.,
*Significantly different from Normal groups ( p<
**Significantly different from high cholesterol diet
Shishoo CJ, The biology and chemistry of
hyperlipidemia, Bioorganic & Medicinal
Chemistry, 2007, 15(14); 4674-4699.
Basis of Therapeutics, McGraw-Hill, USA, 10
edition, 2001; 971-1002.
Saravanan VS, Antihyperlipidemic activity of
Camellia sinensis leaves in Triton WR-1339
induced albino rats, Phcog Mag., 2008, 4(13);
Tao Che, Shou-Xun Zhao, Oleanane saponins
from Gymnema sylvestre, Phytochemistry,
Rajpal V, Standardization Of Botanicals,
herbs, Eastern Publishers, India, Vol-I, 2002;
Nirali Prakashan, Pune, 12
Edition, 2004; 149-
Estimation of the concentration of LDL
cholesterol in plasma, without use of the
preparative ultra- centrifuge. Clin Chem, 18;