expression of apomixis in Paspalum notatum. Sexual Plant Reproduction13, 243-249.
Ranker T (1994) Evolution of high genetic variability in the rare Hawaiian fern Adenophorus
periens and implications for conservation management. Biological Conservation70, 19-
Richards AJ (2003) Apomixis in Flowering Plants: An Overview. Philosophical Transactions:
Biological Sciences358, 1085-1093.
Roche D, Hanna W, Ozias-Akins P (2001) Is supernumerary chromatin involved in gametophytic
apomixis of polyploid plants? Sexual Plant Reproduction13, 343-349.
Rossetto M (2001) Sourcing of SSR markers from related plant species. Plant genotyping: the
DNA fingerprinting of plants, 211–224.
Roy SK (1953) Embryology of Eugenia jambos L. Current Science8, 249-250.
Roy SK (1961) Embryology of Eugenia fruticosa L. Proceedings of the National Academy of
Sciences, IndiaSection B 31, 80-87.
Sahai R, Roy SK (1962) Polyembryony in Eugenia heyneana Duthie. Science and Culture28,
Samadi S, Mavarez J, Pointier JP, Delay B, Jarne P (1999) Microsatellite and morphological
analysis of population structure in the parthenogenetic freshwater snail Melanoides
tuberculata: insights into the creation of clonal variability. Molecular Ecology8, 1141-
Silvertown J (2008) The Evolutionary Maintenance of Sexual Reproduction: Evidence from the
Ecological Distribution of Asexual Reproduction in Clonal Plants. International Journal
of Plant Sciences169, 157-168.
Singhal V, Gill B, Bir S (1985) Cytology of woody species. Proceedings of the Indian Academy
of Sciences: Plant Sciences94, 607-618.
Soltis PS & Soltis DE (2000) The role of genetic and genomic attributes in the success of
polyploids. Proceedings of the National Academy of Sciences 97(13), 7051-7057.
Stebbins GL, Jr. (1941) Apomixis in the Angiosperms. The Botanical Review7, 507-542.
Van Der Hulst RGM, Mes THM, Falque M, et al. (2003) Genetic structure of a population
sample of apomictic dandelions. Heredity90, 326-355.
Van Dijk P, Van Damme J (2000) Apomixis technology and the paradox of sex. Trends in Plant
Van Puyvelde K, Van Geert A, Triest L (2009) ATETRA, a new software program to analyse
tetraploid microsatellite data: comparison with tetra and tetrasat. Molecular Ecology
1.2. Threatened species: population size and diversity 5
2. Materials and Methods 12
The Magenta Lilly Pilly (Syzygium paniculatum), endemic to a narrow strip along the New South Wales coast, is currently listed as vulnerable at both state and national levels. At present management of the species focuses on minimizing currently known threats, such as weed invasion, while little is known about the reproductive biology of the species. S. paniculatum is the only recorded polyembryonic Australian species of Syzygium; polyembryony being the development of multiple (and often asexual) embryos in one seed. Nuclear microsatellite markers were used to investigate the genetic outcome of polyembryony on the reproductive and population biology of the species focusing particularly on the population located on The Entrance Peninsular. Low within-population diversity was found, with low heterozygosity levels and a low level diversity indices when compared to other rare or rainforest species. Multiple embryos from single seeds were found to be identical to the mother. Multiple embryos germinated and survived but one seedling was always significantly taller than all others in the seed but was not considered sexual. It was concluded that the rare S. paniculatum is an apomictic clonal species with extremely low genetic diversity.
1.1. Syzygium paniculatum: a brief description
1.2 Threatened species: population size and diversity
1.3 Polyembryony, clonality and Syzygium paniculatum
1.4 Clonality, fitness and Syzygium paniculatum
1.5 Ploidy in Syzygium paniculatum
1.6 Applicability of nuclear microsatellite markers
1.7 Project outline
2.1 Obtaining and analyzing diversity data
2.1.3 Ploidy identification
2.2 Obtaining and analyzing germination and reproduction data