B. Patra, L. Kuttippurathu, D.
Cook, J.B. Hoek and R. Vadigepalli. Thomas Jefferson Univ.
B691
1161.6 Hepatic steatosis and severe coagulopathy
precede premature death in GCN2 null mice treated with
asparaginase.
G.J. Wilson, P. She, E.T. Mirek, R.C. Wek and
T.G. Anthony. Rutgers Univ. and Indiana Univ. Sch. of Med.
B692
1161.7 Cystathione-
g-lyase deficiency protects
mice from galactosamine/LPS-induced acute liver failure.
K.
Shirozu, K. Tokuda, E. Marutani, D. Lefer, R. Wang and F.
Ichinose. Massachusetts Gen. Hosp. and Harvard Med. Sch.,
Charlestown, Emory Univ. Sch. of Med. and Lakehead Univ.,
Canada.
B693
1161.8 Roux-en-Y gastric bypass surgery prevents
diet-induced hepatic steatosis and endoplasmic reticulum
stress in the liver of obese rats.
J.D. Mosinski, M. Pagadala,
H. Huang, O. Dan, H. Shimizu, E. Batayyah, P.R. Schauer,
S.A. Brethauer and J.P. Kirwan. Cleveland Clin. Fndn.
B694
1161.9 Characterization of the hepatic stellate cell
populations in carbon tetrachloride liver rat through Golgi-Cox
method.
M.J. Gómez Villalobos, R. Giles, I. Carreon, S. Vidrio
and V. Chagoya De Sanchez. Autonomous Univ. of Puebla,
Mexico and UNAM, Mexico City.
1162. MECHANISMS OF INTESTINAL NUTRIENT,
WATER AND ELECTROLYTE TRANSPORT
Poster
t
ue
. 7:30
am
—B
oston
C
onvention
& e
xhiBition
C
enter
,
e
xhiBit
h
alls
a-B
Presentation time: 12:45
pm
-3:00
pm
B695
1162.1 Roles of RSK1 in LPA5R-induced NHE3
regulation.
B.K. Yoo and C.C. Yun. Emory Univ.
B696
1162.2 Intact colonic K
+
secretion in KCNMB2 knockout
mice.
C.K. Larsen, H.A. Praetorius and J. Leipziger. Aarhus
Univ., Denmark.
B697
1162.3 GIP regulates Pept1 via Akt and EPAC signaling
pathways in intestinal epithelial cells.
S. Coon, J. Schwartz
and S. Singh. Boston Univ. and VA Boston Healthcare Syst.
B698
1162.4 Luminal acid-associated post-prandial
epithelial hypoxia maintains the expression of iron absorptive
DMT1 and Dcytb in rat duodenum.
Y. Akiba, I. Kaji and J.D.
Kaunitz. UCLA Med. Sch., West Los Angeles VA Med. Ctr. and
Brentwood Biomed. Res. Inst., Los Angeles.
B699
1162.5 Role of GLUT5 and ketohexokinase in fructose-
induced, acute increases in intestinal active Ca transport.
P.
Tharabenjasin, V. Douard, N. Krishnamra, J. Zuo, R.J.
Johnson and R.P. Ferraris. UMDNJ-New Jersey Med. Sch.
and Mahidol Univ., Thailand.
B700
1162.6 Role of metabolism in fructose-induced GLUT5
regulation.
C.R. Patel, V.R. Douard and R. Ferraris. UMDNJ,
Newark.
B701
1162.7 Mechanisms underlying modulation of
SLC26A3 activity by Lactobacillus acidophilus-secreted
soluble factors.
A. Borthakur, S. Priyamvada, A. Kumar, T.
Gujral, A.A. Natarajan, W.A. Alrefai and P.K. Dudeja. Univ. of
Illinois at Chicago and Jesse Brown VA Med. Ctr.
B702
1162.8 Farnesoid X receptor activation inhibits colonic
epithelial CFTR expression—a new target for antidiarrheal
therapy?
M.S. Mroz, N. Keating, J.B.J. Ward, S. Amu, R.
Sarker, P.G. Fallon, M. Donowitz and S.J. Keely. Royal Col.
of Surgeons in Ireland, Trinity Col. Dublin and Johns Hopkins
Univ. Sch. of Med.
B703
1162.9 Leucine and methionine share a Na/K-
dependent amino acid transporter in shrimp hepatopancreas.
A. Duka and G.A. Ahearn. Univ. of North Florida.
B704
1162.10 The G protein-coupled bile acid receptor,
TGR5, is expressed on colonic epithelial cells and regulates
ion transport.
J.B.J. Ward, M.S. Mróz and S.J. Keely. Royal
Col. of Surgeons in Ireland.
B705
1162.11 Regulation of human intestinal SLC26A3
expression by hepatocyte nuclear factors.
A. Kumar, A.
Alakkam, A. Borthakur, A.N. Anbazhagan, S. Saksena,
R.K. Gill, J. Malakooti, W.A. Alrefai and P.K. Dudeja. Univ. of
Illinois at Chicago and Jesse Brown VA Med. Ctr.
PHYSIOLOGY TUESDAY
409
T
U
E
1163. MEMBRANE TRAFFICKING AND SECRETION IN
DIGESTIVE EPITHELIA
Poster
t
ue
. 7:30
am
—B
oston
C
onvention
& e
xhiBition
C
enter
,
e
xhiBit
h
alls
a-B
Presentation time: 12:45
pm
-3:00
pm
B706
1163.1 Short duodenal acid exposure elicits protective
HCO
3
-
secretary response via Slc26a3, Slc26a9 and CFTR
activation and NHE3 inhibition.
U.E. Seidler, A.K. Singh, Y. Liu,
B. Riederer, R. Engelhardt, B.K. Thakur and M. Soleimani.
Hannover Med. Sch., Germany and Cincinnati Med. Sch.
B707
1163.2 Involvement of store-operated cAMP signaling
in the regulation of colonic secretory function.
J.M. Nichols, I.
Maiellaro, J. Abi-Jaoude, S. Curci and A.M. Hofer. VA Boston
Healthcare Syst./Harvard Med. Sch., West Roxbury.
1164. NEUROTRANSMISSION AND CA
2+
SENSITIZATION IN GASTROINTESTINAL
SMOOTH MUSCLES
Poster
t
ue
. 7:30
am
—B
oston
C
onvention
& e
xhiBition
C
enter
,
e
xhiBit
h
alls
a-B
Presentation time: 12:45
pm
-3:00
pm
B708
1164.1 NANC neuron mediated relaxation of murine
gastric fundus smooth muscle: phosphorylation of MYPT1 on
T696 (ROK site) and S695 (PKG site) are mutually exclusive.
G. Pfitzer, M.S. Hunger, S. Puetz, D. Metzler, L.T. Lubomirov
and A.V. Somlyo. Univ. of Cologne, Germany and Univ. of
Virginia.
B709
1164.2 Inhibition of RhoA/Rho kinase pathway and
contraction by endogenous and exogenous hydrogen sulfide
in gastrointestinal smooth muscle.
A.D. Nalli, S. Rajagopal,
S. Mahavadi, J.R. Grider and K.S. Murthy. Virginia
Commonwealth Univ.
B710
1164.3 Pharmacologically-induced alterations in
gastrointestinal motility affects regional colonic microbial
assemblage.
K. Touw, Y. Wang, E. Huang, V. Leone and E.
Chang. Univ. of Chicago.
1165. RENIN-ANGIOTENSIN SYSTEM
Poster
t
ue
. 7:30
am
—B
oston
C
onvention
& e
xhiBition
C
enter
,
e
xhiBit
h
alls
a-B
Presentation time: 12:45
pm
-3:00
pm
B711
1165.1 The dissociation of the soluble prorenin
receptor in plasma and urine of diabetic patients.
D.Y. Arita, C.
Luffman, C.R.T. Bourgeois, T. Theti and M.C. Prieto. Tulane
Univ. and Fed. Univ. of Sao Paulo.
B712
1165.2 Effects of a (pro)renin receptor blocker on
weight gain in obese mice.
P. Tan, Z. Shamansurova, C.
Michel, T. Nguyen, P. Schiller, J. Gutkowska and J. Lavoie.
Univ. of Montreal, CRCHUM and IRCM.
B713
1165.3 Dexamethasone differentially modulates the
human angiotensinogen gene (hAGT) expression and blood
pressure in transgenic mice containing -6A and -6G haplotype
of the hAGT gene.
V.G. Pandey, S. Jain and A. Kumar. Univ.
of Toledo.
B714
1165.4 Changes in angiotensin II-induced vasomotor
function from newborn to senescence: correlation with
expression of AT1 and AT2 receptors.
Z. Vamos, P. Cseplo,
Z. Bátai, O. Torok, I. Ivic, R. Matics, J. Hamar and A. Koller.
Univ. of Pecs, Hungary.
B715
1165.5 ACE2 activation ameliorates endothelial
function in the right ventricles of pulmonary hypertensive rats.
G.H. Sapp, J. Abbatematteo, C. Cole, V. Shenoy, M. Katovich,
M. Raizada and J. Muller-Delp. Univ. of Florida.
B716
1165.6 Increased ACE expression and iNOS protein
levels in the aorta of 2-month-old Syrian cardiomyopathic
hamsters.
N. Cruz, J. Quidgley, L. El Musa, J. Garcia, G.
Torres, J. Miranda and M.J. Crespo. Univ. of Puerto Rico Sch.
of Med.
B717
1165.7 Effects of chronic swim stress on endocrine
and cardiac function in angiotensin AT1a receptor knockout
mice.
N.K. Almahroug, A. Alhajoj, M. Alghamri, M. Morris
and R.L. Pohlman. Wright State Univ.
B718
1165.8 Angiotensin II receptors modulate nitric oxide
effects on renal sodium handling in conscious lambs.
A.E.
Vinturache and F.G. Smith. Univ. of Calgary, Canada.
B719
1165.9 Ang-(1-7) induced MAS1 receptor-mediated
angiogenesis in the rat microvasculature.
B.R. Hoffmann,
T.J. Stodola, J.R. Wagner and A.S. Greene. Med. Col. of
Wisconsin.
B720
1165.10 Transgenic mice containing two haplotypes
of human angiotensin receptor type 1 show differential gene
expression and regulation of blood pressure.
S. Jain, V.G.
Pandey and A. Kumar. Univ. of Toledo.
B721
1165.11 Production of angiotensin within the SFO is
sufficient to increase ERK1/2 and CREB activity in the SFO
and PVN.
J. Coble, J. Grobe, M. Cassell and C.D. Sigmund.
Univ. of Iowa.
B722
1165.12 Renin gene expression is regulated by
chicken ovalbumin upstream promoter transcription factor
II.
P. Lachmann, S. Mayer, M. Roeser, S. Ishii, J.M. Suh, S.
Harlander, M. Desch, C. Brunssen, H. Morawietz, S.Y. Tsai,
M-J. Tsai, B. Hohenstein, C. Hugo and V.T. Todorov. Univ. of
Technol. Dresden, Univ. of Regensburg, Germany and Baylor
Col. of Med.
B723
1165.13 Human prorenin induces Ang II-independent
pressor response in neuron-specific human (pro)renin receptor
transgenic mice.
H. Peng, W. Li, C.D. Sigmund and Y. Feng.
Tulane Univ. and Univ. of Iowa.
B724
1165.14 Anti-hypertensive effect of chronic ICV
angiotensin-(1-7) in (mRen2)27 transgenic rats is blocked by
Mas antagonist.
L.M. Kangussu, R.A.S. Santos and M.J.
Campagnole-Santos. Fed. Univ. of Minas Gerais, Brazil.
B725
1165.15 Mineralocorticoid receptor antagonism exhibits
comparable BP lowering efficacy in Dahl salt-sensitive rats with
high versus low renin and aldosterone tone.
X. Ma, L. Contino,
O. Price, D. Shuster, L. Xu, J. Xue and J. Metzger. Merck Res.
Lab., Rahway. NJ.
B726
1165.16 Angiotensin II stimulates renin synthesis and
secretion in mouse collecting duct M-1 cells via a PKC alpha-
mediated cAMP stimulation mechanism.
L. Liu, L.S. Lara, A.A.
Gonzalez, C.R.T. Bourgeois, D.M. Seth and M.C. Prieto.
Tulane Univ. Sch. of Med. and Fed. Univ. of Rio de Janeiro.
TUESDAY PHYSIOLOGY
410
B727
1165.17 Loss of cilia increases angiotensin II mediated
apical angiotensin-1-receptor localization in collecting duct
cells.
T. Saigusa, Y. Dang, T.A. Morinelli and P.D. Bell. Med.
Univ. of South Carolina and VA Med. Ctr.
B728
1165.18 Discovery of new renal ang II processing
enzyme activity using mass spectrometry and gene deletion
mouse models.
N. Grobe, K.M. Elased, E.S.B. Salem, S.B.
Gurley, F.S. Ong, K.E. Bernstein, A.H. Schmaier and M.
Morris. Wright State Univ., Duke Univ. and Durham VA Med.
Ctrs., Cedars-Sinai Med. Ctr. and Univ. Hosps. Case Med. Ctr.,
Case Western Reserve Univ.
B729
1165.19 Phosphoproteomic analysis of signaling
responses to angiotensin II in proximal tubules of NHE3-
deficient mice.
X.C. Li, E. Miguel-Qin, G.E. Shull and J.L.
Zhuo. Univ. of Mississippi Med. Ctr. and Univ. of Cincinnati Col.
of Med.
B730
1165.20 Complementation of juxtaglomerular cell
granulation in ren-1d null mice by human renin.
C. Buckley,
R. Nelson, M. Sharp, S. Flemming, C. Kenyon, L. Mullins, D.
Steppan and J. Mullins. Univ. of Edinburgh, Univ. of Dundee,
U.K. and Univ. of Regensburg, Germany.
B731
1165.21 Markers of acute cardiovascular inflammation
induced by angiotensin II in a murine model.
A.B.C. Santana,
T. Souza-Oliveira, V. Barauna, L. Souza, M.C. Irigoyen, L.
Campos, J.E. Krieger and S. Lacchini. Univ. of São Paulo.
B732
1165.22 Angiotensin II receptor signaling modulates
vascular smooth muscle sensitivity to nitric oxide in an
adiposity-specific manner in healthy adults.
D.D. Christou,
M-H. Hwang, A.E. Walker, M. Zigler and D.R. Seals. Univ. of
Florida and Univ. of Colorado Boulder.
B733
1165.23 Acute mineralocorticoid receptor blockade
leads to decreased vascular endothelial function in healthy
older adults.
H.K. Kim, M-H. Hwang, J-K. Yoo, M.J. Luttrell,
T.H. Meade, M.W. English and D.D. Christou. Univ. of Florida
and Texas A&M Univ. Hlth. Sci. Ctr.
Join Us Next Year!
Experimental Biology 2014
April 26 – 30
San Deigo, CA
American Association of Anatomists (AAA)
The American Physiological Society (APS)
American Society for Biochemistry and Molecular Biology (ASBMB)
American Society for Investigative Pathology (ASIP)
American Society for Nutrition (ASN)
American Society for Pharmacology and Experimental Therapeutics (ASPET)
For further information:
www.experimentalbiology.org
PHYSIOLOGY TUESDAY
411
W
E
D
WEDNESDAY, APRIL 24
Pathology
W151
1166.10 Increased oxidative stress with reduced
responsiveness of circulating leukocytes following pulmonary
metal-rich particulate matter exposure.
J.M. Antonini, P.C.
Zeidler-Erdely, S-H. Young, T. Hulderman, M.L. Kashon, J.K.
Gu, R. Salmen-Muniz, T.G. Meighan, J.R. Roberts and A.
Erdely. NIOSH, WV.
W152
1166.11 Increased expression of ER stress genes
in patients with limited cutaneous systemic sclerosis and
pulmonary arterial hypertension.
S. Lenna, A.G. Farina, V.
Martyanov, R.B. Christmann, T.A. Wood, H.W. Faber, R.
Scorza, M.L. Whitfield, R. Lafyatis and M. Trojanowska.
Boston Univ. Sch. of Med., Geisel Sch. of Med. at Dartmouth
and Univ. of Milan.
W153
1166.12 Intranasal administration of IL-37 attenuates
bleomycin-induced experimental lung injury/fibrosis.
S.W. Park,
C.R. Kim, E.H. Lee, J.H. Kim, J.S. Park, J.H. Lee, A.S. Jang,
D.J. Kim, S-t. Uh, Y.H. Kim and C-s. Park. Soonchunhyang
Univ. Bucheon Hosp., Soonchunhyang Univ. Seoul Hosp. and
Soonchunhyang Univ. Cheonan Hosp., South Korea.
W154
1166.13 Leukocyte-specific protein 1 regulates
neutrophil migration in acute lung inflammation.
P.K. Le
Nguyen, S. Channabasappa, L. Liu and B. Singh. Western
Col. of Vet. Med. and Col. of Med., Univ. of Saskatchewan.
W155
1166.14 Administration of PNS on collagen level in
tissue of lung fibrosis in mice.
S. Xiaofang, J. Niu, F. Duan, C.
Huang and J. Wang. Beijing Univ. of Chinese Med. and Hebei
Univ., China.
W156
1166.15 Asbestos increases mitochondrial Ca
2+
and
H
2
O
2
generation in THP1 cells.
A.J. Ryan and B.A. Carter.
Univ. of Iowa and Iowa City VA Med. Ctr.
W157
1166.16 Evidence of donor epithelial type 2 cell
engraftment in bleomycin-injured murine lung after airway
delivery.
P.M. Wang and W.J. Martin II. NICHD, NIH.
W158
1166.17 Chlorpyriphos induces lung inflammation and
alters response to E. coli lipopolysaccharide challenge.
A.A.
Chougule, R. Sethi, D. Schneberger, R.S. Brar, J.P.S. Gill
and B. Singh. Sch. of Animal Biotechnol. and Sch. of Publ.
Hlth., Guru Angad Dev Vet. and Animal Sci. Univ., India and
Univ. of Saskatchewan.
W159
1166.18 Alternaria alternata aeroallergens evoke
ATP release and IL-33 secretion from normal and asthmatic
bronchial epithelial cells.
S.M. O’Grady, P.J. Maniak, C.
Lancto, N. Shivappa and H. Kita. Univ. of Minnesota, St. Paul
and Mayo Clin.
1166. ADVANCES IN NEOPLASTIC AND NON-
NEOPLASTIC PULMONARY PATHOLOGY
Poster
W
eD
. 7:30
am
—B
oston
C
onvention
& e
xhiBition
C
enter
,
e
xhiBit
h
alls
a-B
Presentation time: 11:00
am
-12:00
pm
W142
1166.1 Capsaicin: a novel dietary therapeutic agent in
human small cell lung cancers.
C.A. Stover, C.M. Crabtree,
A.M. Dom, J.K. Lau, K.C. Brown, B. Shiflett, T.R. Witte, W.E.
Hardman and P. Dasgupta. Joan C. Edwards Sch. of Med.,
Marshall Univ.
W143
1166.2 Comparative microscopic study of human
and rat lungs after exposure to welding fume.
J.M. Antonini,
J.R. Roberts, T.G. Meighan, D. Schwegler-Berry and R.R.
Mercer. NIOSH, Morgantown, WV.
W144
1166.3 Complementary roles of KCa3.1 channels
and
b1-integrin in the regulation of alveolar epithelial repair. A.
Girault, R. Bastien, N.T.N. Trinh, A. Prive, E. Maille and E.
Brochiero. Univ. of Montreal Hosp. Res. Ctr.
W145
1166.4 Subsets of fibroblasts show resistance to
apoptosis independently of their interstitial lung disease origin.
M. Selman, V. Vicens, C. Mendoza, M. Montaño, C. Ramos, Y.
Romero and M. Molina. Natl. Inst. of Resp. Dis., Mexico City,
Univ. Hosp. of Bellvitge, Barcelona and UNAM, Mexico City.
W146
1166.5 Pro-inflammatory role of beta2-adrenoceptor
signaling in human airway epithelial cells in response to IL-13.
N.A. Al-Sawalha, I. Pokkunuri, R.A. Bond and B.J. Knoll.
Univ. of Houston.
W147
1166.6 Differentiation of induced pluripotent stem cells
into epithelial cells that express membrane transport proteins
involved in transepithelial Na
+
and Cl
-
transport.
N.A. Zaidman,
J.R. Dutton, A. Mortari and S.M. O’Grady. Univ. of Minnesota,
St. Paul and Minneapolis.
W148
1166.7 Impact of P. aeruginosa bacterial filtrate on
CFTR channels and bronchial epithelial repair.
N.T.N. Trinh,
C. Bilodeau, É. Maillé and E. Brochiero. CHUM, Univ. of
Montreal.
W149
1166.8 The role of hyaluronic acid in SEB-induced
acute lung inflammation.
C.M. Castillejo, O. Uchakina, C.
Bridges and R. McKallip. Mercer Univ. Sch. of Med.
W150
1166.9 Myeloid depletion of SOCS3 enhances LPS-
induced acute lung injury through CCAAT/enhancer binding
protein delta pathway.
H. Gao, C. Yan and X. Wang. Brigham
and Women’s Hosp., Harvard Med. Sch. and Tianjin Union
Med. Ctr., China.
412
Pharmacology and Experimental Therapeutics
1168. NATURAL PRODUCTS II — INFLAMMATION
AND ANGIOGENESIS
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