Additional file 1 Cellular responses induced by Cu(II) quinolinonato complexes in human tumor and hepatic cells

Additional file 1

Cellular responses induced by Cu(II) quinolinonato complexes in human tumor and hepatic cells.

Zdeněk Trávníček^1§, Ján Vančo¹, Jan Hošek¹, Roman Buchtík², Zdeněk Dvořák³

¹Department of Inorganic Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, 17. listopadu 1192/12, CZ–771 46 Olomouc, Czech Republic, [e-mail], phone [phone]. Fax [phone]

²Department of Inorganic Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, CZ–771 46 Olomouc, Czech Republic

³Department of Cell Biology and Genetics, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Šlechtitelů 11, CZ–783 71 Olomouc, Czech Republic

Table of Contents:

Figure S1. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A549 cell line.	2
Figure S2. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the HeLa cell line.	3
Figure S3. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the G361 cell line.	4
Figure S4. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A2780 cell line.	5
Figure S5. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A2780cis cell line.	6
Figure S6. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the LNCaP cell line.	7
Figure S7. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the THP-1 cell line.	8
Figure S8. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the human hepatocytes HH1.	9

Figure S1. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A549 cell line. The cells were plated at 96-well dishes and cultured according manufacturer instructions. The tested compounds were applied to the cells for 24 h in concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed as IC₅₀  SD (μM). The symbol (*) denotes significant differences of IC₅₀ values (p < 0.05) for 16 as compared to cisplatin. The full symbols (■) and () indicate that the compound 5, and 6, respectively, showed significant differences in the IC₅₀ values (p < 0.05) to those compounds marked with the corresponding empty symbols (□) for 2 and (○) for 14.

Figure S2. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the HeLa cell line. The cells were plated at 96-well dishes and cultured according manufacturer instructions. The tested compounds were applied to the cells for 24 h in concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed as IC₅₀  SD (μM). The symbol (*) denotes significant differences of IC₅₀ values (p < 0.05) for 16 as compared to cisplatin. The full symbols (■) and () indicate that the compound 5, and 6, respectively, showed significant differences in the IC₅₀ values (p < 0.05) to those compounds marked with the corresponding empty symbols (□) for 13 and (○) for 13.

Figure S3. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the G361 cell line. The cells were plated at 96-well dishes and cultured according manufacturer instructions. The tested compounds were applied to the cells for 24 h in concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed as IC₅₀  SD (μM). The symbol (*) denotes significant differences of IC₅₀ values (p < 0.05) for 16 as compared to cisplatin. The full symbols (■) and () indicate that the compound 3, and 4, respectively, showed significant differences in the IC₅₀ values (p < 0.05) to those compounds marked with the corresponding empty symbols (□) for 5, and (○) for 1, 5 and 6.

Figure S4. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A2780 cell line. The cells were plated at 96-well dishes and cultured according manufacturer instructions. The tested compounds were applied to the cells for 24 h in concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed as IC₅₀  SD (μM). The symbol (*) denotes significant differences of IC₅₀ values (p < 0.05) for 16 as compared to cisplatin. The full symbols (■) and () indicate that the compound 3, and 4, respectively, showed significant differences in the IC₅₀ values (p < 0.05) to those compounds marked with the corresponding empty symbols (□) and (○) for 5-6.

Figure S5. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A2780cis cell line. The cells were plated at 96-well dishes and cultured according manufacturer instructions. The tested compounds were applied to the cells for 24 h in concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed as IC₅₀  SD (μM). The symbol (*) denotes significant differences of IC₅₀ values (p < 0.05) for 16 as compared to cisplatin. The full symbol () indicates that the compound 4 showed significant difference in the IC₅₀ values (p < 0.05) to compound 2, marked with the corresponding empty symbol (○).

Figure S6. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the LNCaP cell line. The cells were plated at 96-well dishes and cultured according manufacturer instructions. The tested compounds were applied to the cells for 24 h in concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed as IC₅₀  SD (μM). The symbol (*) denotes significant differences of IC₅₀ values (p < 0.05) for 16 as compared to cisplatin. The full symbols (), (■) and () indicate that the compound 4, 5, and 6, respectively, showed significant differences in the IC₅₀ values (p < 0.05) to those compounds marked with the corresponding empty symbols () for 1, (□) and (○) for 13.


Figure S7. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the THP-1 cell line. The cells were plated at 96-well dishes and cultured according manufacturer instructions. The tested compounds were applied to the cells for 24 h in concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed as IC₅₀  SD (μM). The symbol (*) denotes significant differences of IC₅₀ values (p < 0.05) for 16 as compared to cisplatin. The full symbols (#) (), (■) and () indicate that the compound 2, 4, 5, and 6, respectively, showed significant differences in the IC₅₀ values (p < 0.05) to those compounds marked with the corresponding empty symbols (#) and () for 1, (□) and (○) for 13.

Figure S8. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the human hepatocytes HH1. The cells were plated at 96-well dishes and cultured according manufacturer instructions. The tested compounds were applied to the cells for 24 h in concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed as IC₅₀  SD (μM). The symbol (*) denotes significant difference (p < 0.001) between the IC₅₀ values for 2, 46 as compared to cisplatin. The symbol (>) shows that the measured compounds were found non-toxic up to the concentration limit.