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Poster presentation 
99 
METABOLOMIC PROFILING OF Silene SPECIES USING 
UHPLC-MS ANALYSIS 
N. Z. Mamadalieva 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
Silene 
is a genus of flowering plants in the family Caryophyllaceae. Containing 
approximately 700 species, it is the largest genus in the family.
It is distributed mainly 
in the Northern Hemisphere, but some species also occur in Africa and South America.
Phytochemical investigations of the genus 
Silene 
have led to the isolation of several 
phytoecdysteroids, triterpenes, terpenoids, benzenoids, flavonoids, anthocyanidins, 
nitrogen-containing compounds, sterols, and vitamins which have been reported for 
their significant biological properties.
Silene kuschakewiczii
Regel & Schmalh. is natively present in Uzbekistan, while 
Silene viridiflora
L. is introducing in our country since 2004. Previous phytochemical 
studies of 
S. viridiflora
have isolated and identified several ecdysteroids, lipids, neutral 
substances, carbohydrates, and microelements. 
S. kuschakewiczii
has previously never 
been studied for its chemical content. 
Continuing the search for structurally unique and biologically active compounds in 
Silene 
genus, the aerial parts of 
S. kuschakewiczii
and 
S. viridiflora
were investigated. 
The Ultra High Performance Liquid Chromatography coupled to Electrospray 
Ionization Quadruple Time-of-Flight Mass Spectrometry (UHPLC-ESI-Q-TOF-MS) 
technique is a new approach in the chromatographic separations and has been 
successfully employed for fast, high-resolution separations with required sensitivity. 
The TOF-MS is helpful in the structure elucidation and identification of fragmentation 
patterns of the compounds. The present study aimed to explore the secondary 
metabolites present in crude methanol extracts of 
S. kuschakewiczii
and 
S. viridiflora
by 
UHPLC-ESI-Q-TOF-MS. Due to its high content of secondary metabolites, we have 
developed a simple, rapid, and precise method to characterize all the secondary 
metabolites using UHPLC-MS for the methanolic extracts. The unexplored metabolites 
of 
S. kuschakewiczii
belongs mostly to the class of flavonoids. A total 23 secondary 
metabolites (18 flavonoids, 1 triterpene, 1 steroid, 2 phenolic compounds) from 
S. 
kuschakewiczii
were identified. Results showed that the composition of 
S. viridiflora
was found to be rich in triterpenes and ecdysteroids. A total of 34 metabolites (9 
ecdysteroids, 6 flavonoids and 19 triterpenes) were annotated in the methanolic extract 
of this species. 
Our results clearly indicate that species have totally different metabolite profile, 
despite they belong the same genus. The developed method can be successfully applied 
to annotate and identify metabolites in crude methanolic plant extracts.