Organizing co mmittee

Free amino acids in plant protein of D. ajacis 
Non-essential 
amino acids 
Concentration, mg/g 
Essential amino 
acids 
Concentration, mg/g 
Asp 
10.70 
Tre 
6.70 
Glu 
21.23 
Val 
7.92 
Ser 
5.45 
Met 
2.23 
Gly 
9.58 
Iley 
9.73 
Sis.-n 
12.00 
Ley 
13.55 
Arg 
5.49 
Gis 
4.31 
Ala 
9.89 
Fen 
6.39 
Pro 
5.81 
Liz HCl 
5.14 
Tir 
4.94 
Amount 
85.09 
Amount 
55.97 
According to the results of the above research, for the first time it was found that 
D. 
paradoxun
and 
D. ajacis
plants contain all important aliphatic, aromatic and 
heterocyclic free amino acids. 

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Poster presentation 
102 
NEW C
20
-DITERPEN ALKALOID FROM
Delphinium paradoxun 
 
U.Kh. Kurbanov, M.G. Levkovich, N.I. Mukarramov
 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: [e-mail] , tel. [phone]
Plants of the genus 
Delphinium
, distributed in the flora of Uzbekistan, are adapted to 
grow in the soil of the foothills, steppes and deserts. The plant 
Delphinium paradoxun
is 
widely distributed in the desert zones of Navoi and Bukhara regions, on sandy soils, and 
among saxaul groves. 
Information about the chemical composition of 
D. paradoxun
is not found in the 
literature. In our previous works, diterpene alkaloids - ayaconine, 14-dihydrodelcosine, 
neolin, nominine, and 14-O-benzoyldictyocarpine alkaloids - were isolated from this 
plant, and their structure was determined by physical research methods [1-3]. 
We extracted 0.935 kg of 
D. paradoxun
plant from Navoi region of the Republic of 
Uzbekistan in April-May with 80% ethyl alcohol extraction. A total of 2.876 g (0.31%) 
of chloroform alkaloids was obtained in relation to the dry mass of the plant. 
The sum of the obtained alkaloids was separated into fractions by a multiphase 
rechromatographic method on a silica gel column in a mixture of solvents chloroform-
methanol from 200:1 to 1:1. The isolated fractions were separated into fractions in 
hexane, hexane-acetone, chloroform, a mixture of solvents chloroform-methanol by 
rechromatography on columns with aluminum oxide and silica gel (ratios 1:30 to 1:60). 
As a result, the above alkaloids were isolated. 
Similar fractions were combined and rechromatographed on a silica gel column (1:50 
ratio) using the cyclohexane-acetone system. In a 1:1 solvent mixture of cyclohexane-
acetone, fractions 23-27 obtained the base substance.
The structure of the obtained alkaloid was proved by IR, NMR and mass spectra.
The isolated alkaloid is new and belongs to the hetidine type.