Organizing co mmittee

ISOLATION AND IDENTIFICATION OF FUNGI FROM LICHEN 
PRODUCERS OF NEW ANTIBIOTICS 
G.K. Mamytbekova
1
, D.A. Birimzhanova
3
, M.D. Ashimbayeva
1
, O.A. Kataeva
4
,
 
Ye.M. Suleimen
1,2,5
, T. Tursonjan
1
 
 
1
Nazarbayev University, Kazakhstan, Astana, Kabanbay Batyr ave, 53. 
2
Kazakh University of technology and business, Kazakhstan, Astana, K. 
Mukhamedkhanov st, 37 A. 
3
L.N. Gumilyov Eurasian National University, Kazakhstan, Astana, K. Satpayev st, 2. 
4
V.L. Komarov Botanical institute of the RAS, Russia, Saint Petersburg, Popova 
st, 2. 
5 
Sh. Ualikhanov Kokshetau University, Kazakhstan, Kokshetau, Abaya st, 76 
The search for new fungi from lichens, the study of the properties of new antibiotics 
and the determination of the biological activity of drugs and the development of the 
pharmaceutical industry is relevant [1].
31 samples collected from the Komarovsky Bereg Nature Reserve (60°10ʹ N, 29°47ʹ 
E), Leningrad Region, Russia were studied. The objects of research are obtained lichen 
isolates. Isolates were obtained according to method [2]. 
The antibacterial activity of the fungal isolates was determined in relation to 
E. coli, 
St. aureus, S. marcescens
B-RKM 0059, antifungal activity – 
C. albicans
ATCC 885-
653 RKM-0475. 
Micromycetes with antimicrobial activity were transplanted to a solid medium. All 
fungi were grown at room temperature in a dark place for 25 days. Extraction with ethyl 
acetate, biologically active oil mixtures of different colors were obtained: from light 
yellow to brick red.
The molecular-genetic properties of the 5 most active isolated isolates were 
evaluated by DNA isolation, obtaining a PCR product, followed by sequencing and 
analysis of nucleotide sequences in the GenBank - BLAST program to determine the 
generic and species identification of microorganisms. 
31 species were isolated and 11 active species of fungi from lichens with 
antimicrobial and antifungal properties were selected: 
Desmazirella acicola, 
Chaetomum globosum, Coniochaeta hofmannii, Trichoderma atroviride, Fungal sp, 
Coniochaeta ligniaria, Trichoderma harzianum, Fimeariella Rabenhorstii, 
Coniochaeta sp, Hypocrea lixii, Daldinia loculata. 
In the future, it is planned to isolate pure antibiotics from them and their 
structural identification.

This research has been funded by the Science Committee of the Ministry of 
Science and Higher Education of the Republic of Kazakhstan (Grant No. 
AP19679527)»
Literature 
1.
Sethi S., Kumar R., Gupta S. Antibiotic production by microbes isolated from 
soil // International Journal of pharmaceutical sciences and research – 2013. – Vol. 15. – 
P. 2967-2973. 
2. Lehmann J., Joseph S. Biochar for Environmental Maganement. London. – 2015. 
– 976 p. 

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Oral presentation 
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