Organizing co mmittee

 
 

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Poster presentation 
291 
TOXIC EFFECT OF Achillea millefolium ESSENTIAL OILS
ON Tuta absoluta MEYRICK LARVAE 
 
P.A. Nurmakhmadova, Sh.O. Melieva, K.A. Eshbakova, R.P. Zakirova 
 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: [e-mail] 
The tomato leaf miner 
Tuta absoluta
Meyrick is a dangerous pest of nightshade 
crops. The insect, whose natural range was in the countries of South America, has 
become widespread in Africa and Europe. At the present time, the mining moth was 
registered in neighbouring countries, including Uzbekistan [1]. 
These species have become a major pest of tomatoes, causing huge crop losses. The 
fight against 
Tuta absoluta
is hampered by the insect's rapidly developing resistance to 
chemicals. 
Our research aims is to evaluate the toxic effect of 
Achillea millefolium
essential oils 
on the larvae of 
Tuta absoluta
M. under laboratory conditions. 
Essential oils were obtained from the inflorescences of the 
A. millefolium
plant by 
hydro distillation. Leaves of 
Lycopersicon esculentum
Mill
. cultivar 
Leslie F1
containing tomato moth larvae in the mesophyll were placed in Petri dishes and sprayed 
with various concentrations of essential oil. In the control variant, the leaves were 
sprayed with water, Benzoate Super 10% was used as a reference. The condition of the 
pests was taken into account 24 hours after treatment. The biological effectiveness of 
the extracts was determined by the Abbott formula [2]. 
Experiments have shown that the essential oil of 
A. millefolium
has a toxic effect on 
tomato moth larvae. The use of essential oil in doses of 1 and 0.5% caused 100% death 
larvae both the first and second stages of development. Under the influence of 0.1% 
concentration, the mortality in larvae of the first stage was 89%, the second - 77%.
With a decrease in concentration to 0.01%, the biological efficiency was lower - for the 
studied ages and amounted to 75% and 71%, respectively. Essential oils at 0.001% 
concentration caused 63% death of the larvae of the first stage and 55% of the second 
stage. 
Thus, it can be noted that the essential oil of 
A. millefolium
is toxic to tomato moth 
larvae. The biological effectiveness of 1% and 0.5% concentrations was at the level of 
the insecticide Benzoate Super and amounted to 100%.