Organizing co mmittee

STUDY OF THE INFLUENCE OF BIOLOGICALLY ACTIVE 
SUPPLEMENT «BIFOBALANS AKTIV" IN THE EXPERIMENTAL 
RATS: HEMATOLOGICAL RESEARCH 
 
S.M. Nurmuratova, B.J. Azimova 
 
Tashkent pharmaceutical institute, Tashkent, Uzbekistan 
Recently, dietary supplements have become an increasingly urgent problem, 
including in terms of activity in the health sector. In Uzbekistan, there are few studies 
on the ecology of bifidobacteria in the intestines of the local population, their isolation 
from the composition of the intestinal microflora, obtaining pure cultures, studying the 
characteristics of biology, taking into account environmental environmental factors and 
the characteristics of the type of nutrition of the local population. These studies are 
extremely important for our region due to the wide spread of intestinal dysbacteriosis in 
children and adults. Factors causing deficiency of bifidobacteria are high solar 
insolation, saturation of food products with pesticides, mineral fertilizers, high content 
of heavy metal salts in water, unsystematic use of antibacterial drugs, etc. In this regard, 
the development of new drugs and fermented milk products based on local, highly 
effective strains of bifidobacteria is highly relevant. 
The purpose of this study was to study the hematological parameters under the 
influence of the biologically active food additive “Bifobalans aktiv” produced by 
Private limited company “General Med pharm”, Uzbekistan.
Biologically active food additive “Bifobalans aktiv” is a dry powder from white to 
light beige color, with a weak fermented milk smell. In the experiment used white rats 
weighing 138-160 grams. The experimental rats received biologically active food 
additive “Bifobalans aktiv” at doses of 200, 500 and 1000 mg/kg/day by intragastrically 
injection for 30 days.
At the end of the treatment, animals were sacrificed and the blood was collected for 
hematological and biochemical investigations in four groups of control and biologically 
active food additive “Bifobalans aktiv” at doses of 200, 500 and 1000 mg/kg of this 
experiment. There was no significant difference hemoglobin, red blood cells, 
leukocytes, eosinophils, lymphocytes, monocytes, platelets and segmented cells, color 
index and an erythrocyte sedimentation rate (ESR) in peripheral blood in all 
experimental groups. 
The study of the dynamics of hemoglobin, red blood cells, leukocytes, eosinophils, 
lymphocytes, monocytes, platelets and segmented cells, color index and ESR in 
peripheral blood did not reveal statistically significant differences in the animals of the 
experimental group compared with the control data. 
Based on the results of our own research, it was established that the biologically 
active food supplement “Bifobalans aktiv” for adults produced by General Med Pharm 
LLC (Uzbekistan) does not adversely affect the health status of experimental animals, 
nor does it have cumulative properties.