Organizing co mmittee

SYNERGISTIC EFFECT OF THE STEROID BIOSTIMULANT 
AND FUNGICIDE ON THE PHYTOPATHOGENIC FUNGUS 
Fusarium oxysporum sp. vasinfectum 
 
D.T. Babaeva, N.R. Khashimova, A.A. Akhunov, R.R. Shixnazarov 
 
Institute of Bioorganic Chemistry named after academician A.S. Sadykov, Academy of 
Sciences of Uzbekistan, 83 Mirzo Ulugbek street,
100125, Tashkent, Uzbekistan. 
e-mail: [e-mail]
 
Both biotic and abiotic stress inhibits the growth potential of all crops, and the 
mechanisms and strategies for mitigating these effects are still poorly understood. 
Cotton plants are grown in many fields of our republic, but the areas of plant infection 
with phytopathogenic fungi are expanding, which directly hinders the growth and 
development of cotton. 
Fusarium
fungi mainly cause root rot and wilting diseases in the 
cotton plant, causing a sharp decrease in the volume of cotton production and fiber 
quality. Plant biostimulants based on natural materials have received considerable 
attention by both the scientific community and commercial enterprises especially in the 
last two and a half decades. Many natural-based biostimulants contain biologically active 
compounds that participate in the form of signaling molecules and can directly affect 
plant metabolic processes. It is unclear what effects soil or foliar application of the 
unspecified product will have on plants. Optimizing the use of biostimulants requires 
accurate information about the rate and time. Therefore, using biostimulants with 
unknown dosages and the efficiency of functional compounds in plants requires 
determining what processes lead to them.
C-6580 cotton variety seeds were treated with DAG-1 biostimulator based on 
glycyrrhizic acid of steroidal nature, P-4 fungicide, and their composition with four-fold 
reduced P-4 fungicide. The activities of peroxidase (PO), phenylalanine ammonia-lyase 
(PAL) enzymes, and cellulose content were studied in pretreated 7-day-old cotton 
seedlings and roots under the influence of the cultural liquid of the phytopathogenic 
fungus 
F. oxysporum f. sp. vasinfectum
. According to the results of the study, control 
(water) and 
F. oxysporum f. sp. medium
, it was found that the enzyme activities were 
the most active in samples grown under the influence of DAG-1, DAG-1+P-4 
composition. In samples treated with a complex with a reduced fungicide content, 
separately 
F. oxysporum f. sp. vasinfectum
and fungicide P-4 and its complex with a 
bioregulator did not show PO activity in the root compared to the control, but under the 
influence of DAG-1, the activity of PO and FAL in the shoot and root showed high 
results. The cell wall is considered to be the first barrier that pathogens must overcome 
to colonize plant tissuesThe increase in the contents of cellulose in response to biotic 
stress indicates the activation of protective processes. It was found that in 
F. oxysporum 
f.sp. vasinfectum
fungus medium, the amount of cellulose in the roots of seedlings treated 
with the usual dose of P-4 fungicide was higher than the control. A sharp increase in the 
amount of cellulose indicates the activation of lignin synthesis and the formation of a 
mechanical barrier against phytopathogens due to the synergism of biostimulant DAG-1 
and P-4 fungicide. 
The obtained results confirmed that the drug DAG-1 is universal due to its 
stimulating properties, participation in complex biochemical processes in plants, as well 
as synergistic compatibility with P-4 fungicide. 

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Poster presentation 
327