Organizing co mmittee

THE STRUCTURE OF SAPOLIDE FROM THE AERIAL PART OF 
THE SAPONARIA OFFICINALIS 
 
L.N. Ashurova, A.R. Khurramov, Kh.M. Bobakulov, I.T. Mamatvaliev,
N.Sh. Ramazonov 
 
Institute of the Chemistry of Plant Substances, Academy of Sciences of Uzbekistan, 77, 
Mirzo Ulugbek str., 100170, Tashkent, Uzbekistan. E-mail: 
[e-mail]
Plants of the genus 
Saponaria
L. (family Caryophyllaceae) include about 40 species 
distributed in temperate Eurasia, mainly in the Mediterranean region; of these, six 
species grow in Uzbekistan, including 
Saponaria officinalis
. Previously, carbohydrates, 
triterpene glycosides - saponazides, saponariosides, A-M and four hederagenin 
aglycones, hydroxyhederagenin, gypsogenin and quillaic acid, etc. were isolated from 
the roots of 
S. officinalis
. Alkaloids, ascorbic acid, flavonoids: vitexin, saponarin, 
saponaretin were found in the leaves. In official medicine, preparations of 
S. officinalis
are used as an expectorant, choleretic, diuretic, anti-inflammatory, diaphoretic and 
laxative. In continuation of research on the aerial part of 
S. officinalis
growing in 
Uzbekistan, a new natural saponin named sapolide has been isolated (
1
).
The air-dried aerial part of the plant (1 kg) was crushed and extracted with methanol 
at room temperature and after vacuum evaporation a crude extract (445 g) was obtained, 
which was suspended in H
2
O (5 L) and then successively fractionated with chloroform, 
ethyl acetate, and 
n
-butanol. 
n
-Butanol extract (120 g) was chromatographed on a silica 
gel column (0.03-0.200 mm) in a gradient solvent system CHCl
3
–CH
3
OH (1:0-0:1) and 
as a result 4 fractions A-D (1-4) were obtained. Rechromatography of fraction 1 (56 g) 
on silica gel (0.040-0.063 mm) in the solvent system CHCl
3
-CH
3
OH (50:1-0:1) to 
obtain 10 fractions (A 1.1-1.10). Fractions A 1.1-5-6 (48 mg) were further purified by 
preparative TLC (40:1 CHCl
3
-CH
3
OH) to give compound