IF THE RNA POLYMERASE RANDOMLY TRANSCRIBED DNA, THE CELL WOULD NOT MAKE THE CORRECT POLYPEPTIDE
Rich in A-T
Coding strand
ELONGATION
ONCE THE HELIX IS OPEN, RNA POLYMERASE STARTS BUILDING A SINGLE STRANDED MRNA IN THE 5’-3’ DIRECTION USING RNA NUCLEOTIDES AND COMPLIMENTARY BASE PAIRING RULES.
UNLIKE DNA REPLICATION, THE RNA POLYMERASE DOES NOT REQUIRE A PRIMER TO BEGIN BUILDING THE COMPLEMENTARY STRAND
ONLY ONE STRAND OF DNA IS USED AS A TEMPLATE FOR MRNA SYNTHESIS. THIS IS THE TEMPLATE STRAND.
ONLY ONE STRAND OF DNA IS USED AS A TEMPLATE FOR MRNA SYNTHESIS. THIS IS THE TEMPLATE STRAND.
THE OTHER DNA STRAND THAT IS NOT USED FOR TRANSCRIPTION IS THE CODING STRAND
THIS CODING STRAND IS IDENTICAL TO THE MRNA STRAND EXCEPT THAT IT HAS THYMINE INSTEAD OF URACIL.
NOTE: THE PROMOTER REGION DOES NOT GET TRANSCRIBED.
UNLIKE IN DNA REPLICATION, THERE IS NO QUALITY CONTROL ENZYME FOR THE MRNA TRANSCRIPT
UNLIKE IN DNA REPLICATION, THERE IS NO QUALITY CONTROL ENZYME FOR THE MRNA TRANSCRIPT
ERRORS IN MRNA, HOWEVER, ARE NOT TOO DETRIMENTAL SINCE MULTIPLE MRNA TRANSCRIPTS ARE MADE FOR EACH GENE. THEREFORE, SUFFICIENT AMOUNTS OF PROTEIN WILL BE PRODUCED BY THE CORRECT COPIES.