Organizing co mmittee

Mentha longifolia
(leaves)
 
Test 1 
19,07 
19,16 
3,06 
Test 2 
19,25 
Mentha longifolia
(the waste of leaves)
 
Test 1 
20,50 
20,54 
3,28 
Test 2 
20,58 
Mentha longifolia
(the waste of leaves and flowers)
 
Test 1 
13,25 
13,1 
2,09 
Test 2 
12,95 
Thus, it has been determined that 
Mentha longifolia
leaves collected during the 
growth period (May) are a rich source of protein and the waste after the removal of 
lipophilic and semi-polar fractions, with riching of proteins; can be recommended as a 
valuable fodder source. 
 
Reference 
1. N.Sh. Azimov, L.G. Mezhlumyan, U.Sh. Ishimov, S.F. Aripova, D.A. Narbutaeva, 
Z.A. Khushbaktova, Sh.Kh. Rakhimova. 
Chemistry of Natural Compounds, 
57, 599–
600 (2021). 

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Poster presentation 
123 
Polymolecular Complexes of Protein and Chitosan Bombyx mori 
R.Yu. Milusheva, O.B. Avazova, A.T. Abdurasulov, 
S.Sh. Rashidova 
Institute of Polymer Chemistry and Physics of ASRU, Tashkent, Kadyri St., 7, b 
The production of polymolecular complexes (PMC) of protein and chitosan from 
silkworm pupae 
Bombyx mori
, which are waste products of silk production, which have 
antibacterial activity due to the introduction of chitosan, has been developed. A feature 
of chitosan is not only an increase in the resistance of living organisms, but also in the 
ability to regulate and stimulate digestion, the absorption of feed increases, and the 
growth of organisms is stimulated. This allow the use of PMC as an active additive in 
feed for fish, poultry and livestock, as well as in the pharmaceutical, cosmetic and food 
industries. 
Silkworm pupae are used as a source of raw materials, from which a protein 
hydrolyzate is obtained by deproteinization with sodium hydroxide solution. For protein 
coagulation, an organic acid is added at which the maximum protein precipitation 
occurs. By the method of potentiometric titration of protein solutions with a solution of 
chitosan at the isoelectric point (IEP), polymolecular complexes are obtained. 
The IR spectra of chitosan-protein complexes with different percentages of protein 
(from 0.5 to 5%) showed that the intensity of the characteristic absorption bands of the 
complexes strongly depends on the quantitative content of the complex component. 
The nutritional value of proteins depends on their amino acid composition, in 
particular on the presence of essential amino acids. As part of the isolated protein, 16 
amino acids were identified, nine of which are essential - valine, isoleucine, leucine, 
lysine, arginine, histidine, methionine, threonine, phenylalanine. 
The antibacterial activity of chitosan in various concentrations on the suppression of 
various strains of microorganisms 
in vitro
conditions was studied. It is shown that, 
depending on the concentration, chitosan has an antibacterial effect on microorganisms 
of the staphylococcal group - Staph, Klebsiella, Prot. vulgaris and fungicidal 
microorganisms 
Actinomyces
. It should be noted that with an increase in the 
concentration of chitosan to 1%, an antibacterial effect is also observed on anaerobic 
bacteria 
Ps. aerogenosa
. 
The obtained polymolecular complexes were tested at the Scientific Research 
Institute for the Development of Fisheries (Yangiyul) as part of compound feed (with a 
crude protein content of 33.3%) for various indicators: fish output, survival, feed 
efficiency. In the experiment, an industrial species of fish - 
Cyprinus carpio
, was used. 
During the experiment, the survival rate of fish was 94%, which is 7% higher than the 
control, and PМС also had a positive effect on fish growth and feed ratio. 
Thus, the resulting polymolecular complexes based on protein and chitosan, isolated 
from silkworm pupae, which are waste products of silk production, have antibacterial 
activity for use in feed for fish farming, poultry farming and animal husbandry. 
The study was carried out within the framework of the applied project No. F3-
2019062110 "Development of technology for obtaining preparations based on protein 
and 
Bombyx mori
chitosan for fish farming". 

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Poster presentation 
124