Organizing co mmittee

STUDY OF PROCESS EXTRACTION OF THE CYCLOARTHANE 
TRITERPENOIDS FROM AERIAL PART OF PLANTS OF THE 
GENUS Astragalus 
 
A.A. Janibekov
1
, Sh.B. Jandullaeva
2
, U.M. Abillaeva,
2
O.K. Urazimbetova
2
, G.B. Sotimov
1 
 
1) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
2) Karakalpak State University named after Berdakh, Republic of Karakalpakstan
e-mail: [e-mail] 
 
To develop a technology for obtaining cycloartane triterpenoids from the aerial parts 
of 
Astragalus mucidus, Astragalus turkestanus,
and 
Astragalus macronyx,
the 
technological cycle was studied in stages. This work is devoted to the study of the 
extraction process of the aerial part of plants 
Astragalus 
growing in the Tashkent 
region. Was carried out the extraction of raw materials with ethanol with a 
concentration of 50% to 90%. It was found that 80% ethyl alcohol extracts the largest 
amount of the sum of cycloartane triterpenoids, therefore, we chose 80% ethyl alcohol 
as extraction for raw materials.
To determine the optimal fineness of the particle size, the raw material was crushed 
and sieved through a sieve with different hole diameters. From each batch, 0.5 kg of raw 
material was taken and loaded into the extractors as follows: into the first extractor - 
crushed raw material with a particle size of less than 2 mm, in the second - 2-6 mm, in 
the third - 6-10 mm, in the fourth - 10 -14 mm and not ground raw materials were 
loaded into the fifth extractor. Extraction was carried out similarly to the method 
described above. 
To establish the temperature regime, 0.5 kg of the crushed aerial part of 
A. mucidus, 
A. turkestanus, 
and
A. macronyx
with a particle size of 2-6 mm was loaded into four 
extractors, filled with 80% ethyl alcohol until a “mirror” was formed. Extraction in the 
first extractor was carried out at room temperature, extraction in the second extractor - 
at 37 °C, in the third - at 45 °C, in the fourth - at 70 °C. Extraction at temperatures 
above 70°C was not carried out, because the volatility of ethanol increases sharply. The 
obtain thickened extracts were analyzed. 
It has been established that the process is slow during the extraction of not crushed 
and coarsely crushed raw materials. Cycloartane triterpenoids are extracted faster from 
crushed raw materials with a particle size of 2-6 mm, but the extract is cloudy and 
difficult to clarify. As follows from the experimental results, when the cycloartane 
triterpenoids are extracted with 80% ethanol, the extraction rate increases with 
increasing temperature and the solvent consumption decreases. Extraction at a 
temperature of 70°С accelerates the depletion of raw materials, but the resulting extract 
contains a large amount of accompanying substances. It is difficult to isolate the number 
of cycloartane triterpenoids from such an extract. Satisfactory results were obtained 
when extracting raw materials at room temperature (20-30°C). 
When studying the hydromodule, extraction was carried out using various ratios of 
the extractant - raw material, while the optimal hydromodule was 1:5. 

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Poster presentation 
134