Poster presentation
150
DETERMINATION OF FERULIC ACID IN
Ferula tadshikorum RESIN BY HPLC
N.I. Mukarramov
1
, S.F. Aripova
1
, D.B. Barakayeva
2
1) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent
e-mail: mnuriddin@rambler.ru
2) Tashkent state Agrarian university,
е-mail: barakayevadildora0@gmail.com
The article presents the results of the separation of ferulic acid from the composition
of Ferula tadshikorum plant resin by high-performance liquid chromatography (HPLC)
method. During the research, the composition of the main substances obtained from the
resin of the methanol fraction was determined, a standard sample of ferulic acid was
compared and the results were recorded.
For analysis, 10 ml of the tested resin methanolic solution at a concentration of 1
mg/ml and standard ferulic acid 1 mg/ml were prepared, which were subjected to HPLC
analysis on a Shimadzu chromatograph with a UV detector at a wavelength of 320 nm.
A column with silica gel (5 microns) bounds to octadecylsilane, Supelco C18, 150x4,6
mm, 5 microns, was used as the stationary phase. Elution was carried out with a mixture
of acetonitrile: (water: formic acid 20 ml / 1000 ml) - 20:80 in isocratic mode at room
temperature with an eluent flow rate of 0.5 ml/min.
Fig.1. Chromatogram of ferulic acid from methanolic extract of resin
As a result, ferulic acid was identified on the chromatogram of fractions by the
retention time, which coincided with the retention time of the standard sample of ferulic
acid and was 4.6 minutes.
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