Organizing co mmittee

BIODIVERSITY OF COTTON RHIZOSPHERE BACTERIA 
 
N.A.
 
Sherbekova
1
, Q.T. Normurodova
1
, S.I. Zakiryayeva
2
 
1) National University of Uzbekistan named after Mirzo Ulugbek, 100174, Tashkent, 
Uzbekistan 
2) Institute of Microbiology, AS of Uzbekistan, 100128, Tashkent, Uzbekistan 
 
One of the important issues that need to be solved in the whole world today is to 
provide the world's population with quality food and quality clothing products. 
Cotton plant is grown in a lot of countries around the world. Cotton fiber serves as an 
important natural textile fiber, and also cottonseed is an important source of food and 
oil. 
The interaction of plants with symbiotic and beneficial rhizobacteria microorganisms 
is important in the development of plants because it provides them with appropriate 
nutrition and regulators, protects them from pathogenic microorganisms, and adapts 
them to stressful conditions. It is no secret that in most cases, the qualitative description 
of the arable layer determines the activity of the soil microflora. 
Therefore, the purpose of the research is to study the diversity of cotton rhizosphere 
bacteria grown in saline soils of Syrdarya region in Uzbekistan. 
During the conducted research, cotton rhizosphere samples were brought from 
Gulistan district of Syrdarya region in spring, summer, and autumn seasons and their 
biodiversity was studied. As a result, more than 30 strains of rhizobacteria were 
isolated from cotton rhizosphere. 15 cultures were isolated from the spring season, 10 
from the summer season, and 12 from the autumn season. The morpho-cultural 
characteristics of these cultures were studied and identified using the MALDI TOF 
mass-spectrometry method. 
As a result of studying the size, mobility, shape, and size of colonies, optical 
properties, color, surface layer, edges, structure, and consistency of each isolated 
bacterial strain, it was determined that they belong to the genera 
Enterobacter, Bacillus
and 
Lactobacillus
. At the same time, the strains of rhizobacteria were studied to the type 
using MALDI TOF mass-spectrometry method, and as a result, 3 of the rhizobacterial 
cultures were 
Enterobacter cloacae
and 
Bacillus cereus
, one of them was 
Lactobacillus 
malefermentans, B. wakoensis, B. subtilis, B. megaterium
and 
Cedecea davisae,
and two 
strains were 
B. simplex
, five ones were found to belong to 
B. endophyticus species. 
Enterobacter cloacae
was found as the dominant species in cotton rhizosphere in the 
spring season, while 
B. endophyticus
was the dominant species in the summer season. 
To conclude, more than 30 strains of rhizobacteria were isolated from the cotton 
rhizosphere in spring, summer, and autumn, and as a result of their identification using 
the MALDI TOF mass-spectrometry method, it was found that 3 of them belong to the 
genera 
Enterobacter
, 13 to 
Bacillus,
1 to 
Lactobacillus 
and 
Cedecea.
It was observed 
that Enterobacter cloacae species were abundant in cotton rhizosphere in spring. 
B. 
endophyticus
species were found throughout the season.