Organizing co mmittee

 
 
 

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Poster presentation 
321 
DETERMINATION OF THE TOXICITY
OF Rubia tinctorum ROOT EXTRACT 
 
A.S. Ishmuratova, A.Kh. Islamov 
Institute of Bioorganic Chemistry named after academician A.S. Sodikov of the 
Academy of Sciences of the Republic of Uzbekistan; 100125 Tashkent city Mirzo 
Ulugbek street, 83 
As it is known, it is aimed at studying the biology and chemical composition of 
Rubia tinctorum L plant species, which have been used by people for many years. In 
this case, it is of urgent importance to separate natural medicines from local raw 
materials, to prepare cheap and high-quality drugs that can replace imports, and to 
create the initial raw material bases. Such preparations can be isolated from the 
medicinal plant growing in our country and used in medical practice and in the national 
economy. [1-2]. 
No acute toxicity effects were observed in the above-mentioned animals at the 
studied doses of 500, 1000, 1500, 2000 and 3000 mg/kg of 
Rubia tinctorum L
extrac. 
No animal deaths were observed at the above doses observed throughout the 
experiment. Animals in the experimental groups did not show a decrease in body weight 
when compared to the control group. Based on the obtained results, we can conclude 
that the average lethal dose (LD50) of Rubia tinctorum L extrac once injected into the 
stomach of mice is >3000mg/kg. The obtained results are presented in the table below. 
It can be concluded that the results of the study of the acute toxicity of 
Rubia 
tinctorum L
extrac showed that the median lethal dose (LD50) after a single gastric 
injection in mice was higher than 3000 mg/kg, and the samples were found to belong 
tothe class of non-toxic compounds. 
References: 
1.
Mironov A.N. Guidelines for the study of the general toxic effect of drugs, 
“Guidelines for conducting preclinical studies of drugs” Part I, 2012, Mironov A.N., 
Bunyatyan N.D. et al., 2012, pp. 16-17. 
2.
Sidirov K.K. Toxicology of new industrial chemicals. Classification of substances 
by toxicity K.K. Sidirov/M: Medicine, Issue 3. 1973. 47s.