Organizing co mmittee

 
 

---

Poster presentation 
138 
CONCENTRATIONS OF FREE AMINO ACIDS IN THE
ORGANS OF Capparis spinosa 
 
K.Ch. Kurbonov, J.F. Ziavitdinov, U.J. Ishimov, Sh.J. Fazliddinov 
 
Laboratory of cell technologies of vegetable and plant growing, A.S. Sadykov 
Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences 
 
Caper (
Capparis spinosa L.)
is a common member of the genus 
Capparis
, a 
xerophytic shrub with a remarkable adaptability to harsh environments widely spread in 
the southern Europe, the Mediterranean region, in the Caucasus, India and Central Asia. 
Various organs of the plant are known to contain stachydrine (1.2%), flavonoids (0.32-
0.44%), nitrogenous compounds (23-29%) and ascorbic acid (012-0.15%). The work 
was initiated to comparatively study concentrations of free amino acids in 
Capparis 
spinosa 
species widely spread in southern Aralkum and the one growing in the regions 
by the Zerafshan, a river in the Samarkand region of Uzbekistan.
The crushed samples of the above-ground organ of the plant were extracted by means 
of the distilled water. Proteins and peptides of the extract were precipitated using 10% 
trichloroacetic acid to be centrifuged. The supernatant was dried by lyophilization. To 
determine 
concentrations 
of 
amino 
acids 
in 
the 
dried 
supernatant, 
the 
phenylthiocarbomoyl derivatives of the amino acids were synthetized. The derivatives 
were identified using Agilent 1200 Series Chromatograph (Agilent Technologies, USA) 
on the 75 x 4.6mm Discovery HS C18 column. The findings can be seen in the Table. 
Table.
 
Concentrations of non-essential and essential amino acids in leaves
and roots of 
Capparis spinosa