Organizing co mmittee

 
 

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Poster presentation 
206 
EVALUATION OF THE EFFECT OF BIOGENIC SILVER 
NANOPARTICLES ON WHEAT GROWTH
R. B. Ergashev, L. I. Zaynitdinova, R. N. Juraeva, N. A. Lazutin. 
Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, 
Tashkent, 7B str. A. Kadyri 
Nanosilver is the most studied and used for biosystems. It is mostly used for its 
antimicrobial and antifungal properties. Recently, attempts have been made to use 
biogenic silver nanoparticles in agriculture to improve plant growth and increase yields, 
but this issue is still poorly understood and requires further research.
Plants are a major component of the ecosystem and a critical source of food for 
humanity, so understanding the impact of AgNPs on plant growth and development is 
critical to assess potential environmental risks to food safety and human health. Very 
little is known about the positive or negative effect of nanoparticles on plant cells and 
the plant organism, and these data are very contradictory.
In connection with the foregoing, the search for ways to increase the yield of 
agricultural plants is relevant, and one of the possibilities is the use of nanoparticles 
synthesized by microorganisms. We have conducted studies on the production of 
AgNPs using microorganisms and their application to increase the germination and 
germination of wheat seeds to further increase yields.
Using microorganisms belonging to various genera, silver nanoparticles were 
obtained, which were characterized using UV spectroscopic methods and AFM studies. 
It was established that the studied strains had the ability to synthesize AgNPs of oval 
and spherical shape and size from 5 to 100 nm. The obtained solutions containing AgNP 
were used for presowing treatment of wheat seeds. It has been established that the 
soaking of wheat seeds with biogenic AgNPs increases their germination energy in a 
day up to 52% (in the control - 38%), after 3 days - 98% (in the control sample 82%). 
Also shown is a stimulating effect on the growth of roots and stems of the plant in terms 
of such indicators as the length of the stem and root, weight. The results of stimulation 
of growth processes under the action of nanobiosilver may indicate an increase in 
oxidative phosphorylation and photosynthesis, as well as the mobilization of the plant 
antioxidant defense system.
The maximum increase in the mass of dry matter of the roots was observed when 
seeds were treated with cultures in the presence of silver NPs and amounted to 0.032-
0.034 g, and the aerial part 0.028-0.031 g. In all variants of the experiment, the 
maximum stimulation of biomass accumulation was observed in the roots.
Our studies have demonstrated the positive effect of treating wheat seeds with 
nanoparticles synthesized by microorganisms. When wheat seeds were treated with the 
obtained biogenic nanoparticles, an increase in seed germination, stem height, root 
length, dry matter mass of roots and aerial part of seedlings was observed.