Organizing co mmittee

 
N/A
 
N/A
 
N/A
 
N/A 
C. lunata 
6 
9 
8 
7 
N/A 
S. circinatum 
14 
15 
N/A 
13 
N/A 
Ceratobasidium 
sp
2
N/A 
N/A 
N/A 
N/A 
N/A 
Nigrospora 
sp. 
6 
10 
N/A 
9 
7 
Talaromyces 
sp. 
10 
12 
9 
12 
7
References 
1.
 
Gouda S., Das G., Sen S.K. et al. Endophytes: a treasure house of bioactive 
compounds of medicinal importance. Front Microbiol. 2016. V. 7. P. 1538. 
2.
 
Singh A., Singh D.K., Kharwar R.N. et al. Fungal endophytes as efficient sources 
of plant-derived bioactive compounds and their prospective applications in natural 
product drug discovery: Insights, avenues, and challenges. Microorganisms. 2021. V. 
9(1). P. 197. 

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Poster presentation 
216 
STUDY OF GENE EXPRESSION OF DNA AND HISTONE 
METHYLATION AND DEMETHYLATION ENZYMES IN RAT 
STOMACH CELLS 
I.T. Yakubov, J. Yusufjanova 
 
National University of Uzbekistan named after Mirzo Ulugbek, Tashkent, 
e-mail: [e-mail] 
The study of new genes in gastric epithelial cells by transcriptomic methods makes it 
possible to determine their functions and develop new methods for the diagnosis and 
treatment of gastrointestinal diseases. Until now, the functions of about 10% of the 
genes in the stomach cells remained unknown.
In contrast to DNA mutations, epigenetic modifications are reversible and, hence, 
suitable for pharmacological interventions. Reversible histone methylation is an 
important process within epigenetic regulation, and the investigation of its role in cancer 
has led to the identification of lysine methyltransferases and demethylases as promising 
targets for new anticancer drugs. 
The aim of this work is to identify unknown genes in the rat stomach transcriptome 
and to study the expression of genes of histone methylation and demethylation enzymes 
in various organs and cells of the rat stomach. 
In this work, the rat stomach transcriptome database was used, which were deposited 
for public access to the Gene Expression Omnibus National Center for Biotechnology 
Information database under access numbers GPL1439, GSM30415, GSM30416, 
GSM30417, GSE3518, GSM80287, and GSM80288. 
Earlier, using the method of analysis of oligonucleotide microchips, together with 
scientists from the University of California at Los Angeles, more than 41,372 transcripts of rat 
stomach cells were identified. Of these, the functions of 37,698 (91.12%) transcripts were 
known, and the functions of approximately 3,676 (9.88%) genes remained unknown. The 
names and systematic numbers of 2725 genes from 3676 (74.13%) unknown transcripts in the 
rat stomach transcriptome were identified using the stomach transcriptome database and the 
BLAST-nucleotide program. At the same time, the names of 948 (25.87%) genes in remained 
unknown. The identified genes were classified into several groups: various enzymes, 
small and long non-coding RNAs, ribosomal and mitochondrial proteins, zinc-binding 
domain containing proteins, oncogenic proteins, receptors, cytoskeletal proteins, histone 
methylation and demethylation enzymes, and others. 
For the first time, we determined the gene expression levels of the following 
enzymes: lysine demethylase 1B (Kdm1b), lysine demethylase 2A (Kdm2a), lysine 
demethylase 4B (Kdm4b), methyltransferase-like 11B (Mettl11b), RB-binding protein 
5, histone lysine-methyltransferase complex subunits (Rbbp5), methylictose 
dioxygenase 3 (Tet3). Different isoforms of lysine demethylase had different levels of 
gene expression. Isoforms 1B and 4B showed the highest levels of gene expression 
(8299±1493 and 7448±1266, respectively), while isoform 2a showed lower levels of 
expression (1543±200). The ratio of gene expression levels in highly purified parietal 
and enterochromaffin-like cells did not actually change. 
Further study of the expression of genes and proteins of the enzymes of methylation and 
demethylation of rat gastric histones makes it possible to find out the functions of these 
enzymes in the stomach in various pathologies of the gastrointestinal tract. 

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Poster presentation 
217