Organizing co mmittee

References: 
1. Zakirova R.P. et al. Scientific and Applied research in ICPS for agriculture. Journal of Basic 
and Applied Research. V.2, N4. 2016. 
2. Saidaliev H., Khalikova M.B. Resistance to sucking pests of backcross hybrids 
with the participation of 
G.tomentosum
//Zh.: Agromeridian. Almaty. 2006.-1(2). 
3. P.A. Nurmakhmadova, et al. Evaluation of growth promoting activity of extract of 
cotton plant leaves of the line T104. "Actual problems of the chemistry of natural 
compounds" Scientific conference of young scientists. Dedicated to the memory of 
Academician Sabir Yunusovich Yunusov. Tashkent. 17 march. 2022 y. -P.69. 

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Poster presentation 
293 
OBTAINING OF CALLUS TISSUE 
Ajuga turkestanica
 
AND STUDY ITS ANTIFUNGAL ACTIVITY 
 
R.P. Zakirova
1
, M.A. Tulebaeva, Kh.T. Zairova, A.M. Nigmatullaev
1
 
 
1) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
2) Tashkent Pharmaceutical Institute, 
e-mail: [e-mail]  
The flora of the Republic of Uzbekistan is characterized by the richest gene pool of 
useful wild plant species. Medicinal plants of our country are an important part of 
biological resources. Particularly vulnerable species are endemic and rare plant species. 
Cells cultivated in vitro can serve as an additional source of raw materials for 
obtaining products of secondary metabolism. Studies of the secondary metabolism of 
higher plants and cells in vitro are of great importance not only for the creation of 
modern innovative biotechnologies, but also as models for fundamental research [1]. 
The endemic species 
Ajuga turkestanica (Rgl.) Briq.
families Lamiaceaeis is of great 
interest as a source of biologically active substances. Some drugs have been developed 
from this plant and used in medicine. 
The aim of this work was to obtain callus tissue 
Ajuga turkestanica
and study its 
antifungal activity against 
Fusarium oxisporum Schrf.
and 
Aspergillus niger.
To obtain callus tissue, leaf explants of a vegetative plant 
A. turkestanica, 
brought 
from the places of growth in the period of full maturity (end of July 2022) were used. 
To introduce into culture, standard techniques adopted in biotechnology were used. To 
study antifungal activity, we used 
A. turkestanica
callus tissue cultivated for 5 months 
on Murashige and Skoog medium with the addition of auxin 2.4D - 0.5 mg/l and 
cytokinin BAP - 0.2 mg/l. Spores of the fungi 
Fusarium oxisporum
and 
Aspergillus 
niger
were introduced onto the surface of the nutrient medium of the same composition, 
after which callus explants were planted. 
On the fifth day of counting, sterile zones were observed around the callus, on the 
lawn of the microscopic fungus 
F. oxisporum
the zone of no growth was 0.7 cm, on 
A. 
niger
- 0.5 cm. 
The results obtained indicate the synthesis by callus tissues 
Ajuga turkestanica 
substances with fungal properties.