G=100 2A+3*100=750
2A=450
A=225 5) H=3G H=3*100=
300 Javob: A ____________________________
113. Ma`lum sharoitda 110,5 nm uzunlikdagi DNK malekulasi
BamHI restiriktaza fermenti ta`sirida ABCD bo`laklarga bo`lindi
Hosil bo`lgan barcha DNK malekulasidagi vodorod bog`lar soni
dastlabki vodorod bog`lar sonidan 4% ga kam bo`lsa hosil bo`lgan
barcha DNK malekulasidagi G-S orasidagi vodorod bog`lar sonini
toping.
A)300 B)350
C)282 D)438
Ishlanishi: 1) N=L/0,34 N=110,5/0,34=325 325*2=650
2) BamHI har bir kesgan joyda GATC orasidagi H bog’larni
uzadi. 3+2+2+3=10 G-S=2*3=6 ta A-T=2*2=4 ta
3 joydan kesilgan va ABCD bo’laklarga bo’lingan: 10*3=30
3) 30---4%
x---100% x=(30*100)/5=750
4) 2A+3G=750
2A+2G=650
G=100 2A+3*100=750
2A=450
A=225 5) H=3G H=3*100=300 300-18=
282 Javob: C ____________________________
114. Ma`lum sharoitda 110,5 nm uzunlikdagi DNK malekulasi
BamHI restiriktaza fermenti ta`sirida ABCD bo`laklarga bo`lindi
Hosil bo`lgan barcha DNK malekulasidagi vodorod bog`lar soni
dastlabki vodorod bog`lar sonidan 4% ga kam bo`lsa hosil bo`lgan
barcha DNK malekulasidagi A-T orasidagi vodorod bog`lar sonini
toping.
A)300 B)350 C)282
D)438 Ishlanishi: 1) N=L/0,34 N=110,5/0,34=325 325*2=650
2) BamHI har bir kesgan joyda GATC orasidagi H bog’larni
uzadi. 3+2+2+3=10 G-S=2*3=6 ta A-T=2*2=4 ta
3 joydan kesilgan va ABCD bo’laklarga bo’lingan: 10*3=30
3) 30---4%
x---100% x=(30*100)/5=750
4) 2A+3G=750
2A+2G=650
G=100