INTRODUCTION The longevity of cut flowers and leaves is an important problem today. The post harvest quality of
cut flower is dependent on many factors such as the cultivar, per harvest conditions, stage of harvest and
post harvest environment [Ranwala and et.al, 2002]. The cut flower vase life is usually a week or less and
limited by rapid wilting and bract decollation [Kjonboon, 2005]. The longevity of cut flowers in usually
shorter than that of flowers depending on plant [Kokosa, 1997] several treatments have been tested for their
ability to improve Gladiolus flower opening and vase life. Swart [1986] reported that treating prof. Blaaw,
iris stems with 20 mg/l kinetin and /or 10 mg/l gibberellic acid (GA3) for 3d at 2c prior to dry storage for 1
d at 9c reduced the negative effects of water stress. Lee. et al. [21] found that spring gladiolus bulb with 50
mg/l benzyl adenine (BA), a synthetic cytokines, cither alone or in combination with 50 mg/l GA4 delayed
the flower senescence. The addition of inhibitors of protein synthetic (e.g. cyloheximide) and protease
activity (e.g. diisopropyl-fluorophsphate) to vase water can also extend Gladiolus flower longevity by 1-3d
[Jones,Serek and et.al, 1994] kinetin and BA application would prevent leaf yellowing and also improve
the opening and longevity of cut gladiolus flower. Cytokines and gibberellins have been reported in several
studies to improve the post harvest vase life of many cut flowers, Leaf yellowing of excised Easter lily
leaves was significantly delayed by application of gibberellic acid (GA3) 250 mg/l or benzyl adenine (BA)
50 mg/l [Han, 1995] in this study, we determined the optimal BA concentration, treatment for application
on the vase life and post harvest quality of gladiolus cut flower.