Tez özetleri Astronomi ve Uzay Bilimleri Anabilim Dalı

An Investigation the Relationship Between the Unstable Chromosome Aberrations and the Micronucleus Formation in Lymphocytes After in Vitro Application of Radiation

Radiation induced chromosome damage can be measured by cytogenetic techniques. Chromosome damage measured by the chromosome aberration technique is a reliable method in determining an absorbed radiation dose by cells. But the technique has some disadvantages as scoring is hard and requires skill and experience which of these lead low number of cell counts. The micronucleus technique which also measuring chromosome losses has an easy scoring criteria leading high numbers of cell counts and therefore holds more statistical power.

In this thesis project, the relationship between the results of the micronucleus technique and the chromosome aberrations which are good indicators of radiation damage was investigated after giving radiation doses of 1Gy, 2Gy, 3Gy and 4Gy to peripheral blood lymphocytes of healthy individuals. Increases in the chromosome damage after giving radiation were observed by the two techniques. When the chromosome aberrations and the micronucleus frequencies were compared for an each individual, it was shown a significant relationship between the dicentric chromosome damage and the MN frequency. The relationship between the micronuclei and the free acentric chromosome aberrations was not significant as it was between the dicentrics and micronuclei.

Because of the relationship between the dicentric aberration and the micronucleus frequencies, the micronucleus technique with an easy, short term application and scoring can be used as an alternatif to the chromosome aberration technique.