General microbiology

Volutin granules

Plasmids

Capsule

Spores

Flagella

On examination of a 6-year-old child the doctor noticed greyish film on the child’s tonsils. Microscopy of the smear stained by Neisser method detected there Corynebacterium diphtheria. What morphologic feature was the most indicative for determining the type of the agent?

1. 
   Fence-like position of the agent’s cells
   
2. 
   Spores that exceed cells in diameter
   
3. 
   Localization of the causative agent within macrophages
   
4. 
   Polar placement of volutin granules
   
5. 
   Presence of the capsule
   

A smear from the tonsillar coating of a patient with suspected diphtheria was found to contain blue bacilli with a thickening at the poles. What method of smear staining was used?

1. 
   Leffler
   
2. 
   Burri
   
3. 
   Hins
   
4. 
   Gram
   
5. 
   Neisser
   

Microscopy of smear preparation stained with methylene blue revealed bacilli with clublike expansions on their ends similar to C. diphtheriae. What additional method of staining should be used to verify this assumption?

1. 
   Neisser
   
2. 
   Kozlovsky
   
3. 
   Ziehl-Neelsen
   
4. 
   D. Zdrodovsky
   
5. 
   E. Aujeszky
   

A smear prepared from material obtained from patient with suspected diphtheria contains yellow bacilli with blue grains at their ends. What staining was used in this case?

A. Ziehl-Nielsen
B. Kozlovsky
C. Romanovsky
D. Neisser
E. Loefler

21. 
    There are several cases of children from boarding school suffering from sore throat. Microscopy of tonsil smears stained according to Neisser method has revealed thin yellow bacilli with dark brown grains on their ends placed in the shape of Roman numeral five. What infection can be suspected in this case?
    
    1. 
       Diphtheria
       
    2. 
       Infectious mononucleosis
       
    3. 
       Listeriosis
       
    4. 
       Tonsillitis
       
    5. 
       Scarlet fever
       
22. 
    Specimen of a patient’s sputum was stained with the following dyes and reagents: Ziehl’s solution, methylene blue solution, 5% solutoin of sulfuric acid. What staining method was applied?
    
    1. 
       Ziehl-Neelsen
       
    2. 
       Burri’s
       
    3. 
       Gram’s
       
    4. 
       Peshkov’s
       
    5. 
       Neisser’s
       
23. 
    A consumptive patient has an open pulmonary form of disease. Choose what sputum staining should be selected for finding out the tubercle (Koch’s) bacillus?