Organizing co mmittee

 
 

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Poster presentation 
144 
D-PINITOL FROM Astragalus amygdalinus PLANT 
 
S.Z. Aglamov, M.A. Agzamova 
 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: [e-mail] 
 
Nowadays, the problem of obtaining non-toxic or harmless drugs from natural 
sources has become very urgent. 
Biologically active compounds of plants of the genus 
Astragalus
have a number of 
pharmacological activities. In eastern Turkey, in Anatolia, the aqueous root extract 
Astragalus
is traditionally used against leukemia and as a wound healing agent. Studies 
of plants of this genus have shown that on the basis of the released active glycosides, 
the possibility of creating drugs and dietary supplements is increasing. Pinitol is 
accepted as a hypoglycemic medium and was patented for the first time in USA [1].
The purpose of our research is to phytochemically investigate the 
Astragalus 
amygdalinus
plant.
Astragalus almond, Astragalus аmygdalinus
Bunge 
(Fabaceae
) is native in Central 
Asia. 
During the studies, such methods as extraction (air raw materials were extracted with 
ethanol), CC (column chromatography), IR spectroscopy and NMR, TLC were obtained 
two substances: β-
D
-Glucopyranoside β –sitosterol and 
D
-3-
O
-Methyl-chiro-inositol 
[2]. 
From the plant 
Astragalus amygdalinus
, these compounds are isolated for the first 
time. 
β-
D
-Glucopyranoside β -sitosterol, which was identified with an authentic sample by 
TLC and Rf. 
D
-3-
O
-Methyl-chiro-inositol or 
D
-pinitol: IR of the absorption frequency of the 
functional groups of the compound, ν,
𝑐𝑚
−1
: 3390-3296 (ОН), 1652 (С=О), 1613-1556 
(С=С), 1310-1246 (С–О–С).
NMR 
1
Н (400 MHz, C
5
D
5
N, δ, ppm): 3.81s (3H, OCH
3
), 4.18 t (1H, CH), 4.30 m 
(1H, CH), 4.41m (1H, CH), 4.66 t (1H, CH), 4.78 m (1H, CH), 4.83 m (1H, CH). NMR 
13
C (100 MHz, C
5
D
5
N, δ, ppm,): 85.77 (CH), 74.63 (CH), 74.13 (CH), 73.70 (CH), 
73.03 (CH), 72.23 (CH), 60.71 (OCH
3
). 
Chloroform and butanol fractions were examined and showed no fungicidal and 
growth-stimulating activity.
Studies of this compound from the plant 
A. amygdalinus
are ongoing. 
References: 
1.
Almagambetov A.M., Temirgaziev B.S., Zavarzin I.V., Kachala V.V., Kudabaeva 
P.K., Tuleuov B.I., Adekenov S.M. New prospective herbal source of d-pinitola 
possessing anti-diabetic and hypoglycemic properties. 
Chemistry of plant raw materials
. 
2016. №3. P.84 
2.
Agzamova M.A., Isaev M.I. Cycloartane triterpenoids from 
Astragalus 
pycnanthus
. 
Hacettepe Univ. J. of Pharmacy
. Ankara, 1999. 19 (1), P. 45–55.