Organizing co mmittee

 
 

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Poster presentation 
182 
ANTIMICROBIAL ACTIVITIES OF EXTRACTS OF 
ENDOPHYTIC FUNGI ISOLATED FROM CIRSIUM VULGARE 
 
F.B. Eshboev, A.A. Abdurashidov, Kh.Kh. Dolimov, M. Tojieva,
J.M. Abdurakhmanov, M. Murodova, A. Saydalieva, E.G. Yusupova,
G.A. Piyakina, Sh. S. Azimova 
 
S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of 
the Republic of Uzbekistan, 100170, Tashkent, Mirzo Ulugbek str. 77 
 
Antibiotic resistance is an important issue due to the frequent use of antibiotics for 
treatment common bacterial infections, indicating that we are running out of effective 
antibiotics. Endophytes are known to produce a diverse range of natural products, which 
could prove to be a consistent and successful source of drugs. Thus, the study of 
endophytic microbes has great potential within the pharmaceutical industry. Therefore, 
the aim of the research was study of antibacterial and antifungal activities of the extracts 
of endophytic fungi isolated from 
Cirsium vulgare.
For the isolation of endophytic fungi, the 
Cirsium vulgare
leaves were surface 
sterilized and 
leaves were cut into 0.5 cm × 0.5 cm pieces and placed on agar media 
supplemented with antibiotic and incubated at 28 ± 2 °C until fungal growth was 
initiated. The fungal isolates were cultured into (5 × 250 mL) conical flasks containing 
potato dextrose broth for 15 days at 28 °C with 180 rpm of continuous shaking. After 
that, fungal mycelia were extracted three times with an equal volume of ethyl acetate 
(EtOAc). The EtOAc extracts were evaporated to obtain crude EtOAc extracts. Finally, 
antimicrobial activities of EtOAc extracts of fungal isolates from 
Cirsium vulgare
against 
Staphylococcus aureus 
(ATCC 25923),
Bacillus subtilis 
(RKMUz - 5); 
Pseudomonas aeruginosa 
(ATCC 27879), 
Escherichia coli 
(RKMUz - 221) and the 
yeast 
Candida albicans 
(RKMUz - 247) were carried out. 
In this study, two fungal isolates isolated from 
the 
Cirsium vulgare
leaves and 
antimicrobial activities of their 
EtOAc extracts
were conducted. According to the 
results, the extracts of isolate I, and isolate II were showed appreciable (12-15 mm) and 
pronounced (9-10 mm) activities against Gram-positive Bacteria 
Staphylococcus aureus
and
Bacillus subtilis 
respectively. The extracts were showed appreciable (10-13 mm) 
activities against Gramm negative bacteria 
Pseudomonas aeruginosa
. However, the 
extracts were not showed activities against 
Candida albicans
. Nowadays, we are 
working on the molecular identification of active isolates and the chemical composition 
of the fungal extracts.