Organizing co mmittee

 
 

---

Poster presentation 
186 
COMPONENTS OF THE BAC-TO-BAC® BACULOVIRUS 
EXPRESSION SYSTEM
 
M. Shukurjonov, Sh. Khasanov, J. Abdurakhmanov, O. Ashirov,
S. Gaynazarova, N. Tosheva, S. Sasmakov, Sh. Azimova 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: [e-mail] 
 
Nowadays, the Bac-to-Bac® Baculovirus Expression System facilitates rapid and 
efficient generation of recombinant baculoviruses. Recombinant proteins produced by 
baculovirus expression systems are almost indistinguishable from their natural analogs 
by their functional properties, i.e. they have the correct post-translational modification, 
and at the same time this system is characterized by high quantitative product yield. 
Using insect baculoviruses cells for protein expression is safe for humans or other 
mammals, since they are not pathogenic [1-2]. 
The Bac-to Bac® Baculovirus 
Expression System takes advantage of the site-specific transposition properties of the 
Tn7 transposon to simplify and enhance the process of generating recombinant bacmid 
DNA. 
In our experiments, we started selecting the DH10Bac™ Escherichia coli strain to be 
used as a host for our System component pFastBac™ vector. DH10Bac™ cells contain 
a baculovirus vector (bacmid) with a mini-attTn7 targeting site and a helper plasmid. 
After the pFastBac™ expression plasmid is transformed into DH10Bac™ cells, 
transposition occurs between the mini-Tn7 element in the pFastBac™ vector and the 
mini-attTn7 target site in the bacmid to generate recombinant bacmid. This 
transposition reaction takes place in the presence of transposition proteins provided by 
the helper plasmid. 
This greatly simplifies subsequent research. 
References: 
1. Kajikawa M, Sasaki-Tabata K, Fukuhara H, Horiuchi M, Okabe Y, et al. 
Silkworm Baculovirus Expression System for Molecular Medicine. 2012, 
J Biotechnol 
Biomaterial
S9:005. http://dx.doi.org/10.4172/2155-952X.S9-005.
 
2. Wu X, Kamei K, Sato H, Sato SI, Takano R, et al. High level expression of human 
acidic fibroblast growth factor and basic fibroblast growth factor in silkworm (Bombyx 
mori) using recombinant aculovirus. 
Protein Expr Purif. 
2001, 21: 192-200. 
http://www.ncbi.nlm.nih.gov/pubmed/11162406