Organizing co mmittee

 
 

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Poster presentation 
211 
EVALUATION OF THE ANALGETIC EFFECT OF GLABTAN IN 
THE "HOT PLATE" MODEL 
 
G.G. Rakhmonova, R.N. Rakhimov, Q.R. Baratov, R.Ab. Yakubova, N.A. 
Tagayalieva, N.G. Abdullajanova 
Institute of Bioorganic Chemistry of the Academy of Sciences of the Republic of 
Uzbekistan, Tashkent 100125 st. Mirzo Ulugbek 83. 
e-mail: [e-mail]
 
Polyphenolic compounds occupy a special place in modern medicine due to their 
high pleiotropic efficacy. They are widely distributed in almost all plants and have 
strong antioxidant potential due to their unique molecular structure. The staff of our 
institute isolated a sum of polyphenols from 
Rhus glabra
plants, which was 
conditionally named Glabtan. This drug was chosen for screening biological activity 
due to its high water solubility and low toxicity. 
The purpose of this study was to test the analgesic activity of Glabtan using the Hot 
Plate test.
The Hot Plate test is based on behavioral responses controlled by supraspinal 
structures in response to pain. The experiment was performed on healthy mature female 
white laboratory mice, quarantined for 10-14 days, weighing 20±2,0 g, 6 mice per 
group. Glabtan was injected into the stomach of mice of the 1st, 2nd, 3rd, 4th groups at 
doses of 25, 50, 100 and 200 mg/kg, and the control group was injected with an equal 
volume (0,2 ml) of distilled water. After 1 hour, the animals were placed on a metal 
surface heated to 52°C, surrounded by a transparent cylinder. The time interval from the 
moment the animals were placed on the heated plate to the appearance of such reactions 
as licking and pulling up the hind legs, and jumping was recorded.
In the experiment, the analgesic efficacy of Glabtan at doses of 25, 50, 100 and 200 
mg/kg compared with the control was 25 mg/kg – 34,2%, 50 mg/kg – 91,4%, 100 
mg/kg – 67,8 % and 200 mg/kg - 20% of indicators were revealed. When comparing the 
analgesic efficacy of Glabtan at the doses used in the experiment, the analgesic efficacy 
at a dose of 50 mg/kg was 1,3 times higher than that of 100 mg/kg and 4,5 times higher 
than that of 200 mg/kg (p=0,01). Similarly, when comparing doses of 100 and 200 
mg/kg, pain reduction rates were higher at a dose of 100 mg/kg with a statistically 
significant difference (p=0,05).
The study drug Glabtan at a dose of 50 mg/kg had the highest 91,4%, at a dose of 
100 mg/kg 67,8%, and at a dose of 200 mg/kg 20% had the lowest 20% analgesic 
efficacy. Since the effectiveness of Glabtan at doses of 50 and 100 mg/kg exceeded 
50%, it was concluded that this drug has an analgesic effect. It should be noted that 
Glabtan has a positive effect on pain reduction at relatively low doses.
 
Thus, the Glabtan preparation, consisting of the sum of polyphenols isolated from the 
Rhus glabra plant, has the highest analgesic efficacy of 91,4% at a dose of 50 mg/kg.