Organizing co mmittee

EVALUATION OF THE EFFECT ON ARECOLINE TREMOR 
OF THE VINCANINE ALKALOID AND ITS PIRAZOLINE 
DERIVATIVE, UNDER EXPERIMENTAL CONDITIONS 
 
Yu.R. Mirzaev, E.M. Ruzimov, T.T. Khamroev, Sh.M. Adizov,
B.B. Abduazimov, B.N. Khandamov 
 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent
e-mail: [e-mail]  
 
The indole alkaloid vincanine (norfluorocurarine) isolated from Vinca erecta, a plant 
endemic to Central Asia, is known as a strychnine-like agent and its new derivatives can 
be attributed to thymostenic agents, especially in small doses. It is known that
 
currently, 
although significant progress has been made in the diagnosis of various diseases in 
medical practice, the issue of finding and implementing drugs with high activity has not 
lost its relevance. Therefore, large-scale scientific and practical research on the basis of 
plants is carried out on a global scale in search of substances with high pharmacological 
activity and less harmful to the body. In this regard, the Institute the Chemistry of plant 
substances Academy of Sciences of the Republic of Uzbekistan is conducting research 
on vincanin and its derivative, as well as determining its biological activity. 
The purpose of the study.
 
Study of the vincanine and its derivative study of the effect 
on tremor caused by the introduction of arecoline. 
All the studies were conducted on mongrel laboratory white mice with a body weight 
of 20-22 g, which were kept and kept in standard quarantine conditions for 14 days. 
Effect on the M-cholinergic receptors of the studied substance were studied according to 
the recommendations given in the guides and literature. The vincanine and its derivative 
were administered orally to experimental animals in the form of an aqueous solution in 
doses of 0,1, 1,0 and 10 mg/kg, and distilled water in an appropriate volume to animals 
of the control group. Arecoline was administered at a dose of 10 mg/kg under the skin 
of experimental animals an hour after administration of the test substance. 
The results obtained and their discussion.
 
The results obtained on the basis of the 
conducted studies showed that the studied substances vincanine showed an M-
cholinomimetic effect in all doses and pirazoline showed M-cholinolytic effect in all 
doses, depending on the duration of tremor and salivation. 
Thus, in the studies of the vincanine hydrochloride showed an M-cholinomimetic 
and its derivative showed an M-Cholinolytic effect. A number of research works needed 
to study the neuropsychopharmacological properties of vincanine derivatives.