Organizing co mmittee

 
 

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Oral presentation 
34 
BIOLOGICAL ACTIVITY SCREENING AND FRACTIONATION 
OF Naja Naja oxiana VENOM 
 
N.R. Mukhamedov, M.B. Kayumov, Z.Zh. Mirakhmetova, Sh.Ya. Mirzaakhmedov 
 
Institute of Bioorganic Chemistry, 100125, Tashkent, Mirzo Ulugbek street 83. 
e mail: [e-mail] 
Naja naja oxiana
also called the Central Asian cobra is an endemic species of 
venomous snake in the family 
Elapidae
.
 
Its venom contains mainly peptides and 
proteins (80-90%). Drugs produced from snake venom that are already licensed or in 
development show that unpleasant bioactivity may be converted into a therapeutic for 
the correct condition. Though some drugs were discovered in the cobra venom, there is 
a high possibility of finding new ones relying on the rich chemical constituent of the 
object. 
Taking into account the aforesaid opinion, we aimed to separate and isolate novel 
biologically active low molecular weight peptides from 
Naja oxiana
venom. To reach 
the aim, the following tasks were conducted: 1) biological activity screening of the 
venom; 2) ultrafiltration of the venom; 3) comparative analysis of the fractions obtained 
from the ultrafiltration by HPLC. 
For the screening of the biological activity of the venom, it was dissolved in water 
and tested for antioxidant activity and inhibition of more than 10 types of kits 
corresponding to several diseases like cancer, SARS, HIV, etc. According to the test 
results, 
Naja naja oxiana
snake venom showed the following 3 types of biological 
activities at a concentration of 0.1 mg/ml: (1) on HIV-RT (AIDS) – 100% inhibition; (2) 
p300 (cancer) enzyme – 83% inhibition; (3) the antiradical activity of snake venom was 
80% at the initial time and decreased to 67% at 30 minutes. 
Venom was dissolved in 0.1 M ammonium acetate pH 4 buffer to overcome 
proteolytic hydrolysis of the components. Ultrafiltration was carried out using 
ultrafiltration membranes 10 kDa and 3 kDa sizes. >10 kDa, <10->3 kDa, and <3 kDa 
fractions of the venom were obtained as a result. The fractions were lyophilized and 
stored at -20 
o
C for further experiments. 
HPLC analysis of the venom and its fractions obtained after ultrafiltration was 
carried out using the C-18 column in the gradient mode of MeCN against 0.1 % TFA. 
HPLC profiles of all samples were analyzed using Origin software. The HPLC 
chromatogram of the venom consisted of two parts: 1) the hydrophilic part which was 
not adsorbed well in the column and was detected before 5 minutes; 2) the hydrophobic