Organizing co mmittee

CURRANT (RIBESNIGRUM L), PEPPERMINT 
(FOLIA MENTHAE L). 
 
Victoria Bonacheva 
 
Surgut State University, Russia 
 
The research work is aimed at determining the percentage of bioflavonoids in the leaves of 
lingonberries, currants, peppermint which are growing in the Surgut region of the Khanty-Mansi 
area and comparison with the data presented in the scientific literature. The paper presents the 
calculations, made a comparative analysis and presents the conclusions. Using modern analysis 
methods and calculation formulas, the quantitative content of flavonoids in lingonberry leaves 
(in terms of rutin) was found to be 2.811%, in peppermint leaves - 1.881%, in currant leaves - 
1.400%. Studies were conducted to determine the optimal extractant, which was selected as 
70% C
2
H
5
OH. The results obtained indicate the effectiveness and prospect of isolating 
flavonoids in an individual form from this plant material for their further use, as well as the 
creation of medicines based on these valuable substances. Flavonoids are waste products of 
plants. General distribution in flora is inherent. Interest to flavonoids is very great in view of a 
wide range of biological activity inherent flavonoids.
Quantitative determination of flavonoids 
in the alcohol extract was carried out by differential spectrophotometry based on the 
complexation reaction with AlCl
3. 
To extract flavonoids from dry crushed plant material, 
extraction was performed with 70% ethanol. 5 ml of the extract was placed in a 50 ml 
volumetric flask, 20 ml of 95% C
2
H
5
OH and 4 ml of a 2% solution of AlCl
3 
in C
2
H
5
OH were 
added. After 10 min, a 0.2 ml CH
3
COOH solution was added and 95% C
2
H
5
OH was added. So 
we got solution A. 5 ml of the obtained extract was placed in a 50 ml volumetric flask, 20 ml of 
95% C
2
H
5
OH were added. After 10 minutes, 0.2 ml of a solution of CH
3
COOH and 95% 
C
2
H
5
OH (solution B) were added. After 30 minutes, the optical density of solution A relative to 
solution B was measured on an SF-2000 spectrophotometer in cuvettes with a layer thickness of 
10 mm at an analytical wavelength of 410 nm. At the same time, the optical density of the 
solution of a standard sample of rutin prepared as follows was measured: 0.05 g of rutin was 
placed in a 100 ml volumetric flask and C
2
H
5
OH was added to the mark. 1 ml of the resulting 
solution was placed in a volumetric flask with a capacity of 25 ml and then acted as described 
above (preparation of solutions A and B). The calculation of the number of flavonoids in terms 
of rutin in absolutely dry raw materials was carried out according to the formula:
А
х
а
ст
W
x1
W
x2
V
аст
100 * 100 , % 
А
ст
а
х
V
ах
W
ст1
W
ст2
100 
А
х
- optical density of the test solution; А
ст
 - optical density of the rutin solution
 ; а
х
 - leaf 
weight, g; а
ст
 - rutin weight, g; V
ах
 - aliquot volume of the test solution, ml; V
аст
 - aliquot volume of the 
rutin solution, ml; W
х1
, W
х2
- volumes of volumetric flaks in which solutions were prepared, ml; W
ст1
, 
W
ст2
 - volumes of volumetric flaks for the preparation of a rutin solution, ml.
Using the calculation formula, it was determined that the content of antioxidants - flavonoids 
in the leaves of lingonberry is 2.811%, in the leaves of peppermint is 1.881%, in the leaves of 
currant is 1,400 %.