Organizing co mmittee

EXTRACTION PROCESS OF SKIMMIANINE ALKALOID FROM 
Haplophyllum perforatum PLANT 
R.A. Botirov, Sh.R. Nigmanova, A.Z. Sadikov, Sh.Sh. Sagdullayev 
 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent,
e-mail: [e-mail] 
Haplophyllum perforatum
, which has large reserves in the regions of the Babatag 
mountain ranges of the Republic of Uzbekistan, is rich in alkaloids and has 
antidepressant properties. It was determined to be more than 0,5 % of the plants raw 
material mass during the flowering and fruiting vegetation period of the plant [1,2]. 
In order to create a technology for the production of skimmianine alkaloid from plant 
raw materials on an industrial scale, we conducted scientific research work on the 
extraction process of plant raw materials. The influence of the main factors affecting the 
extraction process - the type of extractant, the extraction time, the extraction 
temperature, and the degree of fineness of raw materials - was studied. The influence of 
the main factors on the process, the interrelationship of the factors was determined 
using the Box-Wilson method of mathematical planning. The amount of skimmianine 
alkaloid in the extracts obtained during the experiments was determined using the 
chromatospectrophotometric method. 
As a result of our research, it was determined that in order to extract skimmianine 
alkaloid from the surface of the plant, it is necessary to grind the raw material into 5-7 
mm size, to use 80% ethyl alcohol solvent as an extractant, and to carry out the 
extraction process at a temperature of 20-30°C. Using the obtained results, the kinetics 
of the extraction process was studied, as a result, it was determined that it is necessary 
to extract the raw materials 6 times - 1 - extraction for 8 hours, 2 - 6 hours, 3.4 - 4 
hours, and 5,6 it was determined that it is appropriate to extract the castings for 2 hours. 
As a result of the conducted experiments, it was possible to extract skimmianin alkaloid 
in the amount of 96% compared to its storage in the raw material of the plant during the 
extraction process. At present, scientific research is being carried out on the extraction 
of skimmianine alkaloid from the extract. 
Thus it is first time developed effective extraction method for plants 
Haplophyllum 
perforatum
References: 
1. Yu.R. Mirzaev, Z.I. Sanoev, A.Z. Sadikov, Sh.Sh. Sagdullayev, A.M. 
Nigmatullayev, Kh.A. Rasulova, N.V. Valiev, A.I. Sanoev Means with psychotropic 
activity 
№
. IAP 06452 // Official bulletin 04/30/2021. 
2. Kh.A. Abdullaeva, I.A. Bessonova, S.Yu. Yunusov / Alkaloids 
Haplophylum 
perforatum
// 
Chemistry of Natural Compounds
1978 y. 
№
. 2. P. 219-223. 

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Poster presentation 
95