Organizing co mmittee

Dicthamnus angustifolius (aboveground part) 
Test 1 
10,30 
10,37 
1,65 
Test 2 
10,44 
Dicthamnus angustifolius (meal after extraction with 96% ethanol) 
Test 1 
13,63 
13,55 
2,16 
Test 2 
13,48 
Haplophyllum perforatum (aboveground part) 
Test 1 
8,52 
8,48 
1,35 
Test 2 
8,44 
 
Ruta graveolens (aboveground part) 
Test 1 
7,81 
7,85 
1,25 
Test 2 
7,90 
Thus, it was determined that some alkaloid-containing plants of the 
Rutaceae
family 
have a moderate protein content. Probably, during the growing season, plants produce a 
minimum amount of protein due to their protective functions from being eaten during 
the flowering period. 
References 
1. Ermakov A.I., Arasimovich V.V. 1982. In: Methods of biochemical research of 
plants M. S.430. 
2. N. S. Azimov, L.G. Mezhlumyan, U. S. Ishimova, S. F. Aripova, D. Narbutaeva 
A., Z. A. Khushbaktova, S.H. Rakhimova. Chemistry of Natural Compounds, 57, 599-
600 (2021). 

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Poster presentation 
119 
PROTEIN SUBSTANCES OF RHIZOMES WITH ROOTS OF 
Aconitum septentrionale AND Aconitum leucostomum PLANTS 
 
Sh.Kh. Rakhimova
1
, Kh.O. Tursunov
2
, L.G. Mezhlumyan
1
, A.Z. Sadikov
1
,
Sh.Sh. Sagdullaev
1 
 
1) S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences 
Republic of Uzbekistan, 100170, Tashkent, M. Ulugbek st., 77 
e-mail: [e-mail] 
2) State Center for Expertise and Standardization of Medicines, Medical Devices and 
Medical Technics
2
 
In the metabolism of plants and humans, alkaloids occupy an important place, being 
products of amino acid metabolism. Alkaloids are one of the first compounds of plant 
origin that drew the attention of pharmacologists to create medicinal preparations based 
on them. A significant amount of alkaloids - up to 4-5% by weight of air-dry raw 
materials contains rhizomes with roots that differ little from plants 
Aconitum 
septentrionaleae
and 
Aconitum loucostomum
. These plants are widely distributed all 
over the world; there are significant areas of growth in North America, Europe and 
Asia. There are large reserves of wild-growing raw materials on the territory of the 
Russian Federation and China.
Based on the alkaloids of these plants, new highly effective medicines with 
antiarrhythmic effects of Allapinin and Axaritmin have been created and introduced into 
medical practice. Based on the alkaloids of these plants, the experimental production of 
the ICPS AS RUz annually produces about one tonne of the substance Allapinin and 
Axaritmin, having processed 300-400 tonnes of plant raw materials. 
The purpose of this work is to study the protein substances of these plants, as well as 
in industrial waste such as liquid water waste and solid waste (meal) for possible 
disposal. 
Determination of protein substances in these samples was carried out by colorimetric 
method using Nessler reagent. Because of the conducted studies of the content of 
protein substances in the roots and rhizomes of 
Aconitum septentrionaleae
and 
Aconitum loucostomum
plants, the value of 10-11% protein from the air-dry mass of 
raw materials was obtained. After processing of vegetable raw materials, the protein 
content is kept in the processing waste (liquid and solid). 
IR spectra of the protein were taken on a Perkin-Elmer 2000 IR Fourier 
spectrophotometer in tablets with KBr. IR (KBr, v
max
, cm
-1
): 3218 (-C(O)NH-), 2926 
(CH
2
asymmetric), 1645, 1538 (NH
2
), 1416 (=C-H deformation. oscillation), 1075, 
1027 intensive peak, (-CO-C), 670 (C
6
H
6
) -CH deformation. oscillation, 612 (O=C-N). 
In order to determine the quality of the isolated protein in the roots of 
Aconitum 
septentrionaleae
, their amino acid composition was analyzed. 
It has been established that proteins contain the entire set of essential amino acids. It 
should also be noted that proteins are dominated by amino acids such as lysine, 
phenylalanine, valine, alanine, histidine and glutamine.

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Poster presentation 
120