Organizing co mmittee

QUANTITATIVE ANALYSIS OF PROTEIN IN LIQUID 
EXTRACT “EXTRADENT" 
 
Sh.S. Yuldasheva
1
, N.A. Yunuskhodzhaeva
1
, N.E. Yunuskhodzhieva
1
,
Sh.Kh. Rakhimova 
2 
 
1) Tashkent Pharmaceutical Institute., Tashkent. Uzbekistan 
2) S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences 
Republic of Uzbekistan 100170, Tashkent, M. Ulugbek st., 77
e-mail: [e-mail]  
The use of standardized extracts improves the quality and greatly accelerates the 
technology of water-alcohol extracts. At the Department of Pharmaceutical Chemistry, 
we have developed a liquid extract from herbs of peppercorn 
(Polygonum hidropiper 
L.),
shepherd's purse 
(Bursa pastoris
), calendula flowers (Calendula officinalis L.) and 
nettle leaves (
Urtica dioica L.).
The raw materials of these plants have been used for 
many hundreds of years in scientific and folk medicine. They are pharmacopoeial 
objects that are used as an anti-inflammatory, hemostatic and vitamin remedy.
The purpose of our study was to study the protein content in the new 
phytopreparation 
"Extradent".
To determine the protein content in the isolated fractions, the aliquot part of them 
was taken into a heat-resistant flask (from 5-10 ml), concentrated sulfuric acid H
2
SO
4
(1.84 g/cm3) was added to the aliquot part of the fraction taken. The flasks were placed 
on a sand bath, setting the temperature equal to 400 
0
C. Distilled water was carefully 
poured into the cooled flasks along the walls and quantitatively transferred to a 
measuring flask with a capacity of 50 ml. An aliquot was taken from a measuring flask, 
after mineralization, to determine the protein content by nitrogen, depending on the 
estimated protein content. With a high nitrogen content in the samples, dilution was 
carried out. Then the solution was neutralized. And added 1 ml of Nessler reagent. 
Solutions in flasks were brought to the mark with water and thoroughly mixed. 15 
minutes after painting, the solutions were colorimetrated on an electro-photocolorimeter 
KFK-3 [1-2]. 
Total protein content in liquid alcohol extract “Extradent” 
Sample 
number 
Proteins, % 
Average protein 
content, % 
Proteinic nitrogen 
content, % 
Test 1 
5,61