Organizing co mmittee

STEP-BY-STEP PRODUCTION CONTROL "DRY EXTRACT 
TRIBULUS" FROM THE AERIAL PARTS OF Tribulus terrestris 
D.K. Mutalova, T.A. Khajibaev, R.M. Khalilov, Sh.Sh. Sagdullaev,
R.G’. Axmadjonova 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: [e-mail] 
We have developed a technology for obtainment and launched the serial production of 
the substance "Dry extract Tribulus" with a content of furostanol saponins of at least 45% 
in terms of protodioscin from the aerial part of 
T. terrestris
. The technology is as follows: 
the aerial part of 
T. terrestris
is extracted six times with 70% ethanol at room temperature, 
insisting for 8 hours. The combined and filtered extract is concentrated to a dry weight 
content in the aqueous extract of 25%. The aqueous extract is treated three times with 
chloroform and then twice with ethyl acetate. Furostanol saponins are extracted four times 
from the purified aqueous extract with butanol. The butanol extract is thickened by 
dissolving with water in a ratio of dry mass and water of 1.0 kg: 10.0 l. Then the aqueous 
solution of saponins is concentrated to a dry residue content of 10%. An aqueous solution 
of saponins is dried in a spray dryer at an inlet temperature of 170℃, an outlet of 90℃, a 
solution feed rate of 70.0 l/h, and a spray head rotation speed of 7500 rpm. 
To control the quality of the substance, appropriate methods were designed: the 
authenticity of the substance was assessed using a qualitative reaction for saponins, the 
identification of impurities was carried out by TLC, the quantitative determination was 
carried out by spectrophotometry, and was developed for step-by-step control TLC and 
spectrophotometry methods. 
The results of the step-by-step control of furostanol saponins according to the above 
technology are given in the table. 
Table 
Technology stages 
Yield of total furostanol saponins, % 
% by weight of 
raw materials 
% of the content in 
raw materials 
The aerial part of 
Tribulus terrestris
United extracts 
Schroth 
Chloroform extract 
Ethyl acetate extract 
Butanol extract 
Residual solution 
Spray dryer losses 
Dry extract Tribulus
2,5 
2,3 
0,2 
0,08 
0,15 
1,68 
0,39 
0,14 
1,54 
100,0 
91,9 
8,1 
3,2 
5,9 
67,2 
15,6 
5,6 
61,6 
It was revealed that with the proposed technology, a large amount of furostanol 
saponins remains in the residual solution after extraction with butanol. During spray 
drying, the loss of the finished product is 10-12% of the loaded mass, which determines 
the loss of furostanol saponins at this stage. 

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Poster presentation 
114