Organizing co mmittee

OPTIMAL TIME FOR HARVESTING LICORICE ROOTS FOR 
THE PRODUCTION OF GLABRIDINЕ AND THE PLACE OF 
COLLECTION FROM THE TERRITORY OF UZBEKISTAN
A.I. Sanoev
1,2
, B.S. Okhundedaev
1
, Sh.Sh. Sagdullaev
1
, I.D. Shamyanov
1
,
R.M. Khalilov.
1 
1) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
2) Tashkent Pharmaceutical Institute, 
е-mail: [e-mail] 
In Uzbekistan, the roots of 
Glycyrrhiza glabra
are mainly used on an industrial scale 
as a source of glycyrrhizic acid and its salts. However, the roots of 
Glycyrrhiza glabra
also contain other biologically active substances, including flavonoids such as glabridin, 
liquiritin, isoliquiritin, liquiritoside, etc. In the plant, the content of flavonoids reaches 
4-6% of the mass of air-dry raw materials. This shows that the roots of Glycyrrhiza 
glabra can be a source of biologically active substances based on flavonoids. 
We have studied the dependence of the amount of isoflovane glabridin in the roots of 
licorice growing in Uzbekistan on the growing season and place of growth. 
The content of glabridin was determined by high performance liquid chromatography 
HPLC (Shimadzu) brand "LC-20", Under the following conditions: 
- column "Supelco" (150x4.6 mm) with a stationary phase C18 with a particle size of 5 
μm; 
- column temperature - 40°С; 
- UV detection - 282 nm; 
- the volume of the injected sample was - 20 µl; 
-mobile phase methanol: acetonitrile 1:1; 
- the flow rate of the mobile phase was 1.0 ml/min. 
The mass concentration of glabridin was determined by the sum of the peak areas 
using the external standard method, which was the standard sample of glabridin (Sigma-
Aldrich). The results of the analysis are presented in the table. 
Table 
Changes in the amount of isoflovane glabridin in licorice roots depending on the 
growing season and place of growth 
№ 
Place of collection of licorice 
roots 
Content of isoflovane glabridin % 
March
August October
December 
1 
Republic of Karakalpakstan 
0,102 
0,105 
0,105 
0,108 
2 
Khorezm region 
0,105 
0,107 
0,108 
0,115 
3 
Syrdarya region 
0,130 
0,142 
0,148 
0,152 
The results presented in the table showed that the amount of glabridin in licorice 
root, collected in October and December from the territory of the Syrdarya region, is 
relatively high. In our further work, the samples of roots collected from this place of this 
period were chosen as an object. 

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Poster presentation 
112