Organizing co mmittee

 
 

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Poster presentation 
148 
QUANTITATIVE DETERMINATION OF THE AMOUNT OF 
CAROTENOIDS IN RAW NETTLE (Urtica dioica) 
 
D.R. Gulyamova, N.A. Yunuskhodjayeva 
 
Tashkent Pharmaceutical Institute, st. Aybek 45, Tashkent, Uzbekistan
 
Recently, interest in medicinal plants and the development of effective, safe and 
affordable medicines based on them has increased significantly throughout the world. 
Herbal remedies are widely used for the treatment and prevention of various diseases in 
medical practice. This is due to such advantages as a mild effect on the human body and 
minimization of side effects, which allows us to recommend over-the-counter 
dispensing of the developed herbal remedies. Despite the rich range of drugs of 
synthetic origin, plant-based drugs do not lose their popularity. In dental practice, for 
the treatment of inflammation in the mouth and gums, more and more preference is 
given to the use of herbal medicine. Phytotherapy is a method of treatment based on the 
use of medicinal plants and complex preparations based on them. Plants synthesize 
aromatic substances, most of which are phenols and their oxygen-substituting 
derivatives, such as tannins, useful for maintaining human and animal health.
Stinging nettle (
Urtica dioica 
L., fam. 
Urticaceae 
- nettle) - a popular medicinal 
plant in domestic and foreign medicine. In our country, nettle leaves are used as a 
hemostatic agent. Abroad, rhizomes with roots of stinging nettle are used as raw 
materials in the production of effective drugs for the treatment of prostate adenoma. 
Stinging nettle is one of the most popular medicinal plants in traditional and folk 
medicine. Preparations from nettle leaves are used as a hemostatic, anti-inflammatory, 
and choleretic agent. At the same time, rhizomes with nettle roots are used abroad as a 
raw material for the production of antitumor drugs ("Prostaforton" and "Bazoton") used 
to treat prostate adenoma and prostatitis. 
Method for determining the content of carotenoids, set out in SP XIV edition, provides 
for multiple extraction of pre-prepared raw materials (analytical sample with a particle size 
of 1 mm) with hexane, followed by filtration and measurement of the optical density of the 
obtained extraction at a wavelength of 450 nm. The calculation of the content of active 
substances is based on the sum of carotenoids in terms of 

-carotene. 
About 1 g of raw material (accurately weighed) is placed in a 100 ml flask with a 
thin section, 30 ml of hexane are added, the flask is closed with a stopper and extraction 
is carried out with occasional stirring for 2 hours. The extraction is filtered through a 
paper filter (“red band”). The test solution is prepared as follows: 2 ml of hexane extract 
is transferred into a volumetric flask with a capacity of 25 ml, the volume is adjusted to 
the mark with hexane and mixed. The optical density of the test solution is measured on 
a spectrophotometer at a wavelength of 450 nm in a cuvette with a layer thickness of 10 
mm. Hexane is used as a reference solution. 
Conclusions
. The method of quantitative determination of the amount of carotenoids 
above the ground part of the nettle has been optimized, which provides for a single 
extraction of raw materials with hexane at a ratio of 1:30 for 2 hours with constant 
stirring. It was studied that the content of the sum of carotenoids above the terrestrial