Organizing co mmittee

DETERMINATION OF DEOXYNIVALENOL AND ZEARALENONE IN FOOD 
PRODUCTS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 
 
A.A. Tojikulov, Kh.Kh. Kushiev, T.A. Djuraev, M.S. Khudoynazarov 
 
Gulistan State University, Gulistan, Uzbekistan 
Identification and monitoring of mucotoxins with mutagenic, teratogenic and 
carcinogenic properties in the food and pharmaceutical industry are topical issues. 
Therefore, the study of concomitant contamination of grain crops with mycotoxins, the 
collection and analysis of data on the degree and frequency of their detection are an 
important basis for assessing the risk to public health and developing measures to 
reduce it. This work provides information on mycotoxin contamination of wheat, corn, 
barley, oats and rye grains in Uzbekistan. These include mycotoxins deoxynivalenol 
(DON), toxin T-2 (T-2), zearalenone (ZON), fumonicins B1 and B2 (fw1, fw2), 
aflatoxin B1 (AFL B1), otatoxin a (OTA), which are often found in plant grains. In this 
study, samples of plant grains (corn, rice, barley and wheat) were analyzed using high-
performance liquid chromatography (HPLC) spectrometric method for the presence of 
DON and ZON damage. DON and ZON are two of the five most important natural 
mycotoxins secreted by 
Fusarium
fungi. According to the results of the analysis, a 
quantitative limit of toxins was established based on the established order. At the same 
time, the limit of the amount is from 40 to 100 µg/ml for grain and from 5 to 50 µg/ml 
for the zone. 
Chromatographic analysis has performed in Agilent 1260 Infinity II Rapid 
Resolution Liquid system. In this case, the pump G7111A 1260 Quat Pump VL, 
vialsampler G7129A 1260 is an autosampler, the UV-VIS array detector G7115A 1260 
DAD and G7121A 1260 FLD were used in Chem Station program. For better 
dissolution of solutions, an ultrasonic bath "Guangdong gt ultrasonic" was used. HPLC 
pump flow rate 0,8 ml/min, thermostat temperature 30°C, DON mobile phase; 
Acetonitrile/water in a ratio of 50:50, ZON mobile phase in a ratio of 70:30 at 0,8 
ml/min, injection volume 5 mcl, DON 210 nm, and ZON 240 nm in DAD detector, 
Infinity Lab Poroshel 20 EC-C18 (150 mm x 4,6 mm, 4 microns (Agilent Technologies 
USA) analytical report on the program” Chemstation". 
It has been observed that the correlation coefficient is greater than 0.99 based on 
hummingbird reference samples. The standard linearity and correlation coefficients 
adopted for the drawn ZON and DON using calibration solutions are defined as 0,99999 
for ZON and 0.99981 for DON (Table 1). In standard samples of the linearity curve of 
the method for ZON at different four levels of 5,10,20 and 25 µg/ml, with a quantitative 
limit for ZON at three levels of 20, 40 and 100 µg/ml, a calibration curve was obtained. 
Based on the results obtained, we have noted that ZON was found only in barley 
grains with a quantitative index of 3.56 µg/ml. While the quantitative DON index of 
rice, corn, and wheat participating in the studies were found in plant grain samples, it 
was noted that they contained 6.8 µg/ml in rice, 174.34 µg/ml in corn and 67.2 µg/ml in 
wheat. This will be cost-effective in safety and quality assurance programs.